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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Test method comparable to guideline study, in accordance with accepted German national standards.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
Cited as Directive 84/449/EEC, C.2 (Acute toxicity for Daphnia)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3-methylbut-2-en-1-ol
EC Number:
209-141-4
EC Name:
3-methylbut-2-en-1-ol
Cas Number:
556-82-1
Molecular formula:
C5H10O
IUPAC Name:
3-methylbut-2-en-1-ol
Details on test material:
- Name of test material (as cited in study report): 3-Methylbuten-2-ol-1 (Prenol)
- Lot/batch No.: B 2391
- Analytical purity: 99 %
Water solubility: > 500 mg/l at 20 °C

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Age of test organism: < 24h

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.6 mmol/l
Test temperature:
20 - 21 °C
pH:
7.7 - 8.0
Dissolved oxygen:
7.75 - 8.45 mg/l
Nominal and measured concentrations:
Nominal test concentrations: 15.6, 31.2, 62.5, 125, 250, 500 mg/l and a control
Details on test conditions:
TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4



TEST MEDIUM / WATER PARAMETERS
- Alkalinity: up to pH 4.3: 0.77 mmol/l
- Conductivity: 620 µS/cm


OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: day: night-regime = 16:8 hours
- Light intensity: artificial light, 5 µE at a wave lenght of 400 - 750 nm


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility  after 0, 3, 6, 24, and 48 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
62.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
144 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Reported statistics and error estimates:
EC values (24 and 48 hours) were calculated according to the procedure of  Spearman-Kaerber.

Any other information on results incl. tables

Results after 24 h were as follows:
EC0   = 125 mg/l
EC50  = 234 mg/l 
EC100 = 500 mg/l

Volatility screening test:
Based on TOC measurements the recovery rate at the end of the exposure  period was 94 % for the daphnia test.
This shows that the test substance concentration remained nearly constant  under the conditions of the eco-toxicity test and therefore the effect  values based on nominal concentrations can be used for the hazard  assessment without limitations.

Applicant's summary and conclusion