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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1992
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: well performed OECD and GLP study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
1,3-dihydro-5-nitro-2H-benzimidazol-2-one
EC Number:
202-282-2
EC Name:
1,3-dihydro-5-nitro-2H-benzimidazol-2-one
Cas Number:
93-84-5
Molecular formula:
C7H5N3O3
IUPAC Name:
5-nitro-1,3-dihydro-2H-benzimidazol-2-one

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no
Details on test solutions:
- Due to limited water solubility no stock solution was prepared. The test substance was directly weighed into the test chamber

Test organisms

Test organisms (species):
activated sludge, domestic

Study design

Test type:
static
Water media type:
not specified
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Test temperature:
T = 20 +/- 2°C
pH:
pH was 7.2 +/- 0,2
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorphenol; conc: 5, 20, 30mg/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: no
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: no
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: no

Any other information on results incl. tables

Control:

No respiration inhibition.

Reference Substance DCP:

At 5mg/L the respiration inhibition was 43,6%.

At 20mg/L the respiration inhibition was 76,9%.

At 30mg/L the respiration inhibition was 84,6%.

Nitrolon:

At 1000 mg/l the arithmetic mean of both test vessels is 9% (7,7 and 10,3%) respiration inhibition.

At 500 mg/l the arithmetic mean of both test vessels is 16,7 (12,8 and 20,5 %) respiration inhibition.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Not toxic against sludge.
Executive summary:

No deviation from the test guideline was observed, the criteria for validity of the study are fulfilled. Result: EC50 is above 1.000 mg/l.

The test substance is therefore classified: not toxic against sludge.