Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Analytical data missing

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: internal company guideline
Deviations:
not specified
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Nako H is aquivalent to p-Phenylenediamine (see NCI)
The test substance was pre-desolved in the test-water and afterwards applicated to the test basins.
pH, O2 and temperature was determined before adding test material and checked after 2, 24, 48, 72 and 96 h

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Leuciscus idus melanotus
Details on test organisms:
ex Paul Egger, 2345 Hohenwestedt

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
de-chlorinated water (activ-carbon filter), hardness 17.2 and 17.0 °d
carbonate hardness 11 and 9 °d (Aquamerck, E. Merck, Darmstadt)
Test temperature:
20 +/- 1°C
temperature was tested directly after adding the substance and 2, 24, 48,72 and 96 h afterwards with Hg-thermometer
pH:
8.1 - 8.7 (Digital-ph-Meter 650, Knick, Elektronische Meßgeräte, Berlin)
Dissolved oxygen:
100 ml air/min, O2-content > 7mg/l
O2 was tested directly after adding the substance, 2, 24, 48,72 and 96 h afterwards with Oxi 39 (Wissenschaftlich-Technische-Werkstätten GmbH, Weilheim, Obb.)
O2-content 7.8 - 8.7 mg/l
Details on test conditions:
light/dark rythm 12/12 h, light from the top, light density directly over the tank approx. 700 Lux
Fishes were added to the water min. 14 days at 20 °C before adding test substance. 65 h before beginning of the test 10 fishes per tank (20l) were added.

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
>= 0.1 - <= 1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: no data
Details on results:
highest tested concentration without mort. 0.1 mg/l at 48 and 96 h
lowest tested concentration on 100 % mort. 1 mg/l at 48 and 96 h
fishes died within 24 h after starting the test.
The group in concentration between 0.01 and 0.1 mg/lg didn't show any difference to control group

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
LC50 = 0.1 to 1 mg/l