Hydrocarbons, C8-10

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

2008

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study was conducted using basic scientific principles. Summary is from published literature.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Review of the toxicology of mineral spirits

Author:

Amoruso M.A., Gamble J.F., McKee R.H., Rohde A.M.

Year:

2008

Bibliographic source:

International Journal of Toxicology 27: 97–165

Reference 2

Reference Type:

publication

Title:

Petroleum hydrocarbon toxicity studies. Ill. Animal and human response to vapors of Stoddard Solvent

Author:

Carpenter C.P., Kinkead E.R., Geary Jr. D.L., Sulivan L.J., King J.M.

Year:

1975

Bibliographic source:

Toxicology and Applied Pharmacology 32: 282-297

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

8 hour exposure

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Mineral Spirits

IUPAC Name:

Mineral Spirits

Details on test material:

Mineral Spirits

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

no data

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

8 h

Concentrations:

0.27, 0.45, 0.79, 1.9, 2.4, 4.6, 8.2 mg/L; 8.2 mg/L; 8.2 mg/L = 1400ppm

No. of animals per sex per dose:

15 animals

Control animals:

yes

Details on study design:

Groups of 15 young male rats inhaled various concentrations, to provide a lethal and a no-ill-effect level for single 8-hr periods. Ten of the fifteen animals were randomly selected for the determination of the lethal and the no-ill-effect levels, based upon a 14-day observation period, and five for gross autopsy and histopathological evaluation immediately following the exposure.

Statistics:

no data

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LC50

Effect level:

> 8.2 mg/L air (nominal)

Remarks on result:

other: 1400 ppm; 1 animal died at this exposure

Mortality:

1 animal died at the 8.2 mg/L exposure

Clinical signs:

other: Their response at this concentration included eye irritation, bloody exudate around the nostrils, and slight loss of coordination. When rats inhaled a measured 4.6 mg/liter (800 ppm), they showed similar signs but no loss of coordination. Inhalation of 2.

Body weight:

Weight changes at the 4.6 and 8.2 mg/L doses. No effects at 2.4 mg/L.

Gross pathology:

no data

Other findings:

none

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

LC50 (4- to 8-h exposures) in rats for mineral spirits is greater than 8.2 mg/L

Executive summary:

Groups of 15 young male rats inhaled various concentrations, to provide a lethal and a no-ill-effect level for single 8-hr periods. Ten of the fifteen animals were randomly selected for the determination of the lethal and the no-ill-effect levels, based upon a 14-day observation period, and five for gross autopsy and histopathological evaluation immediately following the exposure. The LC50 >8.2 mg/liter (1400 ppm), which was maximum concentration attained under the test system conditions.