Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
02/08/1994-04/08/1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report Date:
1994

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Norme Francaise AFNOR T 90 301 de janvier 1983

Test material

Specific details on test material used for the study:
The test solutions were prepared in different ways according to their physicochemical properties:
- Soluble products (powders and liquids): preparation of a test solution at 1 g / l.
- for not soluble liquids: production of a stable emulsion, 1 g/l solution treated for 5 mins with ultrasound (20 kHz, 50 watts)
- for not soluble powders: preparation of a test solution of 1 g of product in 20 ml of DMSO

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
not specified
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20°C +/- 2°C
pH:
2.27

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.4 mg/L

Any other information on results incl. tables

 concentration

 N° of daphnia immobilized after 48 h

Total / 20

 % of immobilisation  pH  O2 mg/l
 0  0    
 solvent control 2%  10    
 0.65  2  10    
 0.9  4  20    
 1.3  8  40    
 1.8  14  70    
 2.5  16  80    
 3.5  20  100 7.93   6.6

EC(I)50 -48h calculated by the computerized method of calculation of PROIBITS (95% confidence interval): 1.40 mg/l (1.20 -1.63 mg/l)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The percentage of daphnia immobilized within 48 hours in the control tubes is 0% and is therefore ≤ 10%.
The conditions are simultaneously satisfied. The results obtained for the 26 products studied can therefore be considered valid.