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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

The toxic effects of the test substance on the oxygen consumption rate of aerobic micro-organisms (activated sludge) after short-term exposure of 180 min was determined according to the OECD 209 (2010). The EC50 was determined to be 170 mg/L and the EC10 was determined to be 20 mg/L. 

Key value for chemical safety assessment

EC50 for microorganisms:
170 mg/L
EC10 or NOEC for microorganisms:
20 mg/L

Additional information

The objective of this key study according to OECD 209 (2010) was to determine the toxic effects of the test substance on the oxygen consumption rate of aerobic micro-organisms (activated sludge) after short-term exposure of 180 min, compared with the blank control. The test was performed in nominal concentrations of 0 (control), 9.5, 30.5, 97.7, 313, 1000 mg/L without correction of the purity. No concentrations were measured. The inoculum was activated sludge from a municipal waste treatment plant of Mannheim, Germany. The sludge concentration was 1.5 g/L and a synthetic medium according to the guideline was used. A reference substance (3,5 dichlorphenol) was used as positive control. The EC50 value of the reference substance 3,5-dichlorphenol was determined to be 11.8 mg/L, which was within the acceptable limits, (5.0 to 30 mg/L) as specified in OECD 209 Guideline. All validation criteria were fulfilled. The EC50 for the test substance was determined to be 170 mg/L and the EC10 for the test substance was determined to be 20 mg/L.