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EC number: 221-117-5
CAS number: 3007-53-2
The toxic effects of the test substance on the oxygen consumption rate of aerobic micro-organisms (activated sludge) after short-term exposure of 180 min was determined according to the OECD 209 (2010). The EC50 was determined to be 170 mg/L and the EC10 was determined to be 20 mg/L.
The objective of this key study
according to OECD 209 (2010) was to determine the toxic effects of the
test substance on the oxygen consumption rate of aerobic micro-organisms
(activated sludge) after short-term exposure of 180 min, compared with
the blank control. The test was performed in nominal concentrations of 0
(control), 9.5, 30.5, 97.7, 313, 1000 mg/L without correction of the
purity. No concentrations were measured. The inoculum was activated
sludge from a municipal waste treatment plant of Mannheim, Germany. The
sludge concentration was 1.5 g/L and a synthetic medium according to the
guideline was used. A reference substance (3,5 dichlorphenol) was used
as positive control. The EC50 value of the reference substance
3,5-dichlorphenol was determined to be 11.8 mg/L, which was within the
acceptable limits, (5.0 to 30 mg/L) as specified in OECD 209 Guideline.
All validation criteria were fulfilled. The EC50 for the test substance
was determined to be 170 mg/L and the EC10 for the test substance was
determined to be 20 mg/L.
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