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EC number: 221-117-5
CAS number: 3007-53-2
In the semi-static immobilization test, young Daphnia magna STRAUSS (< 24 h old) were exposed to the test item for 48 hours according to OECD 202 (2004). The EC50 value was determined to be nominal 0.313 mg test item/L.
In the acute immobilization test with
Daphnia magna (STRAUS), the effects of the test substance with a nominal
loading of 0.046, 0.1, 0.22, 0.46 and 1.0 mg test item/L and a control
(0 mg test item/L) were determined according to OECD 202 (2004) and
Council Regulation (EC) No. 440/2008/C2 (2008) under GLP conditions. The
corresponding mean measured concentrations of the test substance were
determined to be 0 (control), 0.045, 0.095, 0.205, 0.425, 0.975 mg/L.
The study encompassed 4 replicates per concentration and 1 replicate per
control. The number of the organisms per vessel was 5. The mobility of
the Daphnia was determined in a static-renewal (semi-static) 48-hour
test by visual observation after 24 and 48 hours. The renewal period was
24 hours. The samples collected at start and at the end of the test (48
hours) were analyzed via capillary gas chromatography - mass
spectrometry (GC-MS). The EC50 of the reference substance (sodium
chloride) after 48 hours was determined to be 4.78 g test item/L. This
result was within the range of 3.88 – 7.22 g/L and indicates that the
culture of Daphnia magna used in this study was responding normally to
toxic stress. The water pH, temperature and dissolved oxygen were within
acceptable guideline specifications. The toxicity results presented here
were consistent with the results from preliminary tests. Immobilization
(mortality) and sublethal effects were observed daily. The analytically
determined concentrations of the test substance in the test solutions
were within ± 20% of the nominal concentrations, therefore the effect
concentrations can be expressed relative to the nominal concentration
for the evaluation of the test substance. The NOEC was determined to be
0.22 mg test item/L, the Lowest Concentration with 100% immobile was
determined to be 0.46 mg test item/L and the EC50 value was determined
to be 0.313 mg test item/L. All reported results refer to the nominal
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