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Short-term toxicity to aquatic invertebrates

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Description of key information

In the semi-static immobilization test, young Daphnia magna STRAUSS (< 24 h old) were exposed to the test item for 48 hours according to OECD 202 (2004). The EC50 value was determined to be nominal 0.313 mg test item/L. 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
0.313 mg/L

Additional information

In the acute immobilization test with Daphnia magna (STRAUS), the effects of the test substance with a nominal loading of 0.046, 0.1, 0.22, 0.46 and 1.0 mg test item/L and a control (0 mg test item/L) were determined according to OECD 202 (2004) and Council Regulation (EC) No. 440/2008/C2 (2008) under GLP conditions. The corresponding mean measured concentrations of the test substance were determined to be 0 (control), 0.045, 0.095, 0.205, 0.425, 0.975 mg/L. The study encompassed 4 replicates per concentration and 1 replicate per control. The number of the organisms per vessel was 5. The mobility of the Daphnia was determined in a static-renewal (semi-static) 48-hour test by visual observation after 24 and 48 hours. The renewal period was 24 hours. The samples collected at start and at the end of the test (48 hours) were analyzed via capillary gas chromatography - mass spectrometry (GC-MS). The EC50 of the reference substance (sodium chloride) after 48 hours was determined to be 4.78 g test item/L. This result was within the range of 3.88 – 7.22 g/L and indicates that the culture of Daphnia magna used in this study was responding normally to toxic stress. The water pH, temperature and dissolved oxygen were within acceptable guideline specifications. The toxicity results presented here were consistent with the results from preliminary tests. Immobilization (mortality) and sublethal effects were observed daily. The analytically determined concentrations of the test substance in the test solutions were within ± 20% of the nominal concentrations, therefore the effect concentrations can be expressed relative to the nominal concentration for the evaluation of the test substance. The NOEC was determined to be 0.22 mg test item/L, the Lowest Concentration with 100% immobile was determined to be 0.46 mg test item/L and the EC50 value was determined to be 0.313 mg test item/L. All reported results refer to the nominal concentrations.