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EC number: 221-117-5
CAS number: 3007-53-2
In the semi-static short-term toxicity to fish test according to OECD 203 (1992), the 96-hour LC50 of the test substance for Zebrafish (Danio rerio) was determined to be greater than 0.88 mg test item/L (value based on mean initial measured concentration).
The acute toxicity of the test
substance to fish Zebrafish (Danio rerio) was determined according to
OECD 203 (1992) and EU Council Regulation No. 440/2008/C1 (2008) under GLP
conditions. This study encompassed one treatment group for the dose
rates of the test item and one replicate for the control. Each group
contained 7 individuals. The acute toxicity to unfed juvenile Zebrafish
(Danio rerio) was determined in an aerated, semi-static, 96-hour test
under 48 hours renewal conditions. The test fish were observed after 1,
6, 24, 48, 72 and 96 hours test duration for sublethaI effects and
mortality. Dead fish were removed at least once daily and discarded. The
sampIes of the test medium were analyzed via capillary gas
chromatography - mass spectrometry (GC-MS) at start of the test and
after 48 and 96 hours.
The nominal test concentrations based
on the test substance density without a correction for purity were 0
(control), 0.046, 0.10, 0.22, 0.46, 1.0 mg/L and the mean initial
measured concentrations were determined to be 0 (control), 0.04, 0.08,
0.19, 0.36, 0.88 mg/L. The concentrations declined over the 48 h renewal
interval to undetectable levels. Consequently a reliable mean measured
concentration could not be calculated from the analytical measurements.
The initial measured concentrations were consistent between the two
renewal intervals and all individual values were within ± 20 % of the
mean. Test concentrations did not remain within ± 20 % of the nominal or
mean measured values over the test period and this validity criterion
identified by the test guideline was not met. All other test validity
criteria required by the test guideline were met. Reasonable efforts
were taken to maintain stable test concentrations, including
preconditioning the test vessels and using a semi-static exposure.
Excessive test substance loss was not observed in other aquatic studies
(acute Daphnia and algae growth) with this substance. The test substance
loss in this fish test may be due to rapid biodegradation from flora
introduced with the test fish. The water pH, temperature and dissolved
oxygen were within acceptable guideline specifications.
There were no toxic effects observed
in any treatment group during this test. The 96 hour LC50 was determined
to be greater than 1 mg/L based on the nominal concentrations and
greater than 0.88 mg/L based on the initial mean measured
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