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Short-term toxicity to fish

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Description of key information

In the semi-static short-term toxicity to fish test according to OECD 203 (1992), the 96-hour LC50 of the test substance for Zebrafish (Danio rerio) was determined to be greater than 0.88 mg test item/L (value based on mean initial measured concentration).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
0.88 mg/L

Additional information

The acute toxicity of the test substance to fish Zebrafish (Danio rerio) was determined according to OECD 203 (1992) and EU Council Regulation No. 440/2008/C1 (2008) under GLP conditions. This study encompassed one treatment group for the dose rates of the test item and one replicate for the control. Each group contained 7 individuals. The acute toxicity to unfed juvenile Zebrafish (Danio rerio) was determined in an aerated, semi-static, 96-hour test under 48 hours renewal conditions. The test fish were observed after 1, 6, 24, 48, 72 and 96 hours test duration for sublethaI effects and mortality. Dead fish were removed at least once daily and discarded. The sampIes of the test medium were analyzed via capillary gas chromatography - mass spectrometry (GC-MS) at start of the test and after 48 and 96 hours.

The nominal test concentrations based on the test substance density without a correction for purity were 0 (control), 0.046, 0.10, 0.22, 0.46, 1.0 mg/L and the mean initial measured concentrations were determined to be 0 (control), 0.04, 0.08, 0.19, 0.36, 0.88 mg/L. The concentrations declined over the 48 h renewal interval to undetectable levels. Consequently a reliable mean measured concentration could not be calculated from the analytical measurements. The initial measured concentrations were consistent between the two renewal intervals and all individual values were within ± 20 % of the mean. Test concentrations did not remain within ± 20 % of the nominal or mean measured values over the test period and this validity criterion identified by the test guideline was not met. All other test validity criteria required by the test guideline were met. Reasonable efforts were taken to maintain stable test concentrations, including preconditioning the test vessels and using a semi-static exposure. Excessive test substance loss was not observed in other aquatic studies (acute Daphnia and algae growth) with this substance. The test substance loss in this fish test may be due to rapid biodegradation from flora introduced with the test fish. The water pH, temperature and dissolved oxygen were within acceptable guideline specifications.

There were no toxic effects observed in any treatment group during this test. The 96 hour LC50 was determined to be greater than 1 mg/L based on the nominal concentrations and greater than 0.88 mg/L based on the initial mean measured concentrations.