2,2,4-trimethylpentane-1,3-diol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study conducted under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,2,4-trimethylpentane-1,3-diol

EC Number:

205-619-1

EC Name:

2,2,4-trimethylpentane-1,3-diol

Cas Number:

144-19-4

Molecular formula:

C8H18O2

IUPAC Name:

2,2,4-trimethylpentane-1,3-diol

Details on test material:

- Name of test material (as cited in study report): 2,2,4-Trimethyl-1,3-pentanediol or TMPD
- Physical state: white solid
- Analytical purity: 99%
- Purity test date: 2000-11-6
- Lot/batch No.: 021500

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

At test start (time 0), the exposure solutions were prepared, physical parameters were measured, and samples were collected for concentration verification. At test end, (time 48-hours) physical parameters of each exposure solution were measured and samples were collected for analysis.

Test solutions

Vehicle:

no

Details on test solutions:

Replicate exposure solutions (A&B) were prepared at a nominal concentration of 110 mg/L by the addition of the appropriate amount of test substance to a 1000-mL volumetric flask brought to volume with dilution water. The test substance solution was sonicated for approximately 5 minutes to enhance the dissolution of the test substance. After sonication, the flask was inverted several times to ensure mixing. Aliquots (200 mL) of the exposure solution were then transferred to the daphnid test vessels.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Adult Daphnia magna were reared within 100-L culturing tanks located within the Testing Facility. The gravid daphnids used to produce the test animals for the study were obtained from rearing tanks which had been established for at least two weeks. Prior to the study, approximately 100 gravid daphnids were transferred by net into two glass bowls containing diluent water and food. After approximately 24 hours, all of the adult daphnids were removed from the bowls using nets and pipettes and the neonates were used for testing.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

130 mg/L

Test temperature:

The temperatures of the exposure solutions in all vessels ranged from 20-21°C throughout the test.

pH:

The pH values ranged from 8.4 to 8.5.

Dissolved oxygen:

The dissolved oxygen values ranged from 8.7 to 9.0 mg/L.

Nominal and measured concentrations:

110 mg/L nominal and 109.1 geometric mean measured

Details on test conditions:

At test start (time 0), the exposure solutions were prepared, physical parameters were measured, and samples were collected for concentration verification. Ten test organisms were then introduced into each replicate test vessel. Stratified randomization was accomplished by alJocating to each vessel no more than 50% of any one set of test organisms at a time. Observations for signs of immobility and stress were made at times 0, 24, and 48 hours. At test end, (time 48-hours) physical parameters of each exposure solution were measured and samples were collected for analysis.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 109.1 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 109.1 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No immobility was observed in Daphnia in both the control and test substance throughout the exposure.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The acute toxicity of the test substance to Daphnia magna was determined in a 48-hour, static, aquatic effects limit test. The mean analyzed value for the test substance exposure concentration was determined to be 109.1 mg/L. Concentration verification was performed using gas chromatography with flame ionization detection (GC/FID). The test substance exposure concentration was calculated by averaging the geometric means (for replicates A and B) of the test substance analytical results at test start (time 0) and test end (time-48 hours). The 24- and 48-hour EC50 values for Daphnia magna were estimated to be> 109.1 mg/L. The highest tested concentration causing no immobility within the period of the test was determined to be 109.1 mg/L. The daphnids in the dilution water controls exhibited normal behavior and appearance throughout the test. No immobility was observed during this study.