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EC number: 215-222-5
CAS number: 1314-13-2
This study was conducted to determine the photoallergenic potential of a
topically applied product under maximization conditions.
0.2g of the test material or hydrophilic ointment USP (negative control)
was placed onto a 2cm x 2 cm occlusive hypoallergenic patch, then
applied to subject's back. The patch is then applied directly to the
skin of the infrascapular regions of the back. After 24 hours the test
sites are wiped dry with gauze and one site is exposed to 3 Mininimal
Erythemal Dose (MED) of solar simulating radiation while the alternate
site is left nonirradiated. This sequence is repeated for a total of 6
exposures twice weekly.
7 -10 days after the final induction exposure. Similar occlusive
applications are made for 24 hours to a previously unexposed untreated
area of normal skin. The sites are then irradiated with 10.0 Joules/cm2
of UV-A radiation
Observation times for skin reaction were scored 48 and 72 hours post
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