Zinc oxide

Toxicity to other above-ground organisms

Administrative data

Endpoint:

toxicity to other above-ground organisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Effects of dietary zinc level and an inflammatory challenge on performance and immune response of weanling pigs

Author:

Guo-Jun S, Dai-Wen C, Ke-Ying Z, Yu B

Year:

2009

Bibliographic source:

Asian-Australasian J. Anim Sci. 22 1303–1310.

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Dietary toxicity study in pigs

GLP compliance:

no

Remarks:

peer-reviewed publication without information on GLP

Test material

Test material information

Constituent 1

Reference substance name:

Zinc sulphate

EC Number:

231-793-3

EC Name:

Zinc sulphate

Cas Number:

7733-02-0

Molecular formula:

H2O4S.Zn

IUPAC Name:

zinc sulfate

Details on test material:

- Name of test material (as cited in study report): zinc sulphate (ZnSO4.7H2O)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Tissue samples were prepared for analysis by wet digestion with HNO3 and HClO4, The dried, fat-free phalanges were ashed at 550°C for 48 h, and bone samples were then dissolved in 5 ml of 6 N HC1 and diluted appropriately for Zn analysis.

Test organisms

Test organisms (species):

other wild mammal species: pigs

Details on test organisms:

TEST ORGANISM
- Age at test initiation: weaned at 28 d
-weight: 6.76 ± 0.47kg
- Kept according to standard practices: yes (Sichuan Agricultural University Animal Care and Use Committee)

Study design

Study type:

laboratory study

Limit test:

no

Total exposure duration:

21 d

Post exposure observation period:

0 days

Test conditions

Test temperature:

25-28°C

Humidity:

not reported

Photoperiod and lighting:

12 h light:12 h dark were

Details on test conditions:

Housing: 1.28×1.28 m pens (cycle of 12 h light:12 h dark)
Daily food intake per body weight (kg diet /day/ kg bw): 334 (Control), 332 (60 Zn mg/kg), 337 (120 Zn mg/kg)
Control: Basal diet without Zn supplementation
Concentration in control diet (mg Zn/kg diet): 36.75
No of animals (M/F) per dose: SIx replicates per treatment with
four pigs per pen

Nominal and measured concentrations:

dose (mg Zn/kg diet, added): 0, 60, 120 (nominal values)

Results and discussion

Effect concentrations

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 120 mg/kg diet

Nominal / measured:

nominal

Conc. based on:

element (total fraction)

Remarks:

Zn

Basis for effect:

growth

Remarks:

average daily growth

Details on results:

no significantly different effects of different Zn concentrations

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

An unbounded NOEC of ≥120 mg Zn/kg diet (added as ZnSO4) was observed fro growth of pigs.