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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
EPA OTS 797.1400 (Fish Acute Toxicity Test)
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The test substance was added without a carrier solvent to dilution medium at the highest treatment level. This was then stirred for up to 4 hours, and the remaining concentrations prepared by serial dilution. The reported solubility of the test substance was incorrectly stated as 46.13 mg/L. A reliable water solubility test has shown this to be much lower (1.26 mg/L).
Test organisms (species):
Pimephales promelas
Details on test organisms:
TEST ORGANISM
- Common name: fathead minnow
- Source: Aquatic Research Organisms, Hampton, NH
- Age at study initiation: 1-14 days (- Feeding during test: Not fed during test and fed with Artemia nauplii during acclimitisation

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
Moderaely hard re-constituted water with a hardness value of 98 mg/L as CaCO3.
Test temperature:
22°C +/-2°C
pH:
Dilution medium pH value of 8.1
Dissolved oxygen:
Dilution medium DO value of 7.9 mg/L
Nominal and measured concentrations:
Definitive phase: 0, 2.83, 5.67, 11.25, 22.5 and 45 mg/L
Details on test conditions:
TEST SYSTEM
- Type: open
- Material, size, headspace, fill volume: 475 mL polypropylene cups (250 mL fill volume).
- Renewal rate of test solution: No renewal (static)
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Moderately hard re-constituted water formulated from deionised water produced from E-Pure deionization system that is amended with reagent grade chemicals following EPA's Acute Whole Effluent Toxicity Test Methods.
- Total organic carbon: < 1.0 mg/L
- Chlorine: < 0.01 mg/L
- Alkalinity: 72 mg/L as CaCO3
- Conductivity: 341 umhos/cm

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours darkness
- Light intensity: 10-20 μE/m2/s (50-100 ft-c).

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortality (48 and 96 hours).

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0, 0.01, 0.1, 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study
Reference substance (positive control):
not required
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 45 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: In the test report this is stated as the limit of solubility however, the test substance water solubility is less than this value
Details on results:
- Mortality of control: No mortality observed in the controls.
- Test material precipitation observed at all concentrations.
- Effect concentrations exceeding solubility of substance in test medium: Yes; the reported solubility of the test substance was incorrectly stated as 46.13 mg/L. A reliable water solubility test has shown this to be much lower (1.26 mg/L). The results obtained in this study far exceed the solubility of the substance.
Validity criteria fulfilled:
yes
Remarks:
100% control survival
Conclusions:
In a 96 hr acute toxicity study, the 96hr LC50 was above the solubility limit of the test substance in test medium.

Description of key information

In a 96hr acute toxicity study, Fathead Minnow (Pimephales promelas) were exposed to the test substance at concentrations of 0 (control), 2.83, 5.67, 11.25, 22.5 and 45.0 mg/L under static conditions (nominal concentrations).

Key value for chemical safety assessment

Additional information

Due to the low solubility of the test substance in test medium, concentration levels that might be toxic for fish could not be reached (concentrations in any case tested above the limit of aqueous solubility, and no mortality was observed). The 96hr LC50 was above the solubility limit of the test substance in test medium.