Registration Dossier
Registration Dossier
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EC number: 205-769-8 | CAS number: 150-76-5
Toxicity to other above-ground organisms
Administrative data
- Endpoint:
- toxicity to other above-ground organisms
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Even if the species is not currently used in the standardised test, the test conditions are detailed and scientifically acceptable. The GLP are not mentioned.
Data source
Reference
- Reference Type:
- publication
- Title:
- Acute toxicity of substituted phenols to Rana japonica tadpoles and mechanisms-based quantitative structure-activity relationship (QSAR) study
- Author:
- Wang X, Dong Y, Wang L, Han S
- Year:
- 2 001
- Bibliographic source:
- Chemosphere, 44, 447-455.
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Investigation of the toxicity of substances on frog tadpoles in a 24h exposure test.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Mequinol
- EC Number:
- 205-769-8
- EC Name:
- Mequinol
- Cas Number:
- 150-76-5
- Molecular formula:
- C7H8O2
- IUPAC Name:
- 4-methoxyphenol
- Details on test material:
- provided by Department of Chemistry at Nanjing University (analytical purity).
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- no
- Details on preparation and application of test substrate:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
Method: Aerated chloride-free water was used for preparing the stock solutions of chemicals and test dilution.
Control: yes, the aerated chloride-free water without test compounds served as the control.
Evidence of undissolved material: no data.
Test organisms
- Test organisms (species):
- other: Rana Japonica tadpoles
- Details on test organisms:
- TEST ORGANISM
- Common name: Japanese Brown Frog.
- Strain: no data
- Source: naturally fertilized egg masses collected from a permanent pond in Nanjing.
- Age at study initiation: 30-day old.
- Weight at study initiation: 0.09 (+/-) 0.01 g
- Method of culturing: in aerated stream water in the laboratory at room temperature (22-25°C).
- Feeding during test: no, feeding was halted 24h prior to testing.
ACCLIMATION
- Acclimation period: no acclimatation.
Study design
- Limit test:
- no
- Total exposure duration:
- 24 h
- Post exposure observation period:
- none
Test conditions
- Details on test conditions:
- STUDY DESIGN
- Test type: semi-static.
- Test vessel type: open.
- Water media type: fresh.
TEST CONDITIONS:
- Hardness:data not available.
- Test temperature: 22-25°C.
- pH: 6.63
- Dissolved oxygen: data not available.
- Nominal and measured concentrations: no data
Details on test conditions:
TEST SYSTEM
- Test vessel: 1000-mL pyrex beaker containing 500 mL test solution.
- Renewal rate of test solution: every 6 hours.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control / vehicle control: (replicates): 3
TEST MEDIUM / WATER PARAMETERS: aerated chloride-free water. No other information.
OTHER TEST CONDITIONS
- Adjustment of pH: no.
- Photoperiod: 14h light, 10h dark.
- Light intensity: data not available.
EFFECT PARAMETERS MEASURED: mortality.
STATISTICAL ANALYSIS: using non-linear regression in Statistica for Window software (1995). - Nominal and measured concentrations:
- - Spacing factor for test concentrations: a six-step concentration series in geometric grade ranging from no effect to 100% lethal concentration
were set after range finding experiments.
RANGE-FINDING STUDY: yes.
- Results used to determine the conditions for the definitive study: 6 concentrations between 0 and
100% lethality. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 12 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2.371 other: mmol/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 2.371 other: mmol/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- No mortality occurred in the control.
Any other information on results incl. tables
The result can be converted to 24h LC50 = 294 mg /L.
Applicant's summary and conclusion
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