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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Sept-Oct. 1981
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Remarks:
Study performed before GLP was in place and comparable to guidelines.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1981
Report date:
1981

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
GLP compliance:
no
Test type:
other: acute oral median lethal dose

Test material

Constituent 1
Chemical structure
Reference substance name:
Ammonium nitrate
EC Number:
229-347-8
EC Name:
Ammonium nitrate
Cas Number:
6484-52-2
Molecular formula:
H3N.HNO3
IUPAC Name:
ammonium nitrate
Test material form:
solid
Remarks:
granules or crystals
Details on test material:
No further details provided.

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: methylcellulose and Tween 80
Doses:
Range finder: 50, 250, 1250, 5000 mg/kg
Definitive: 2000, 2380, 2830, 3360 mg/kg
No. of animals per sex per dose:
Range finder: 2
Definitive: 5
Control animals:
not specified

Results and discussion

Preliminary study:
The mortalities indicated an LD50 in the range 1250-5000 mg/kg.
Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
2 950 mg/kg bw
Mortality:
Mortality occurred at 3360 (7/10), 2830 (3/10), 2380 (4/10) mg/kg and also 1 animal in 2000 mg/kg.
Clinical signs:
other: Prostration, lethargy and deep rapid breathing were the main clinical signs. All surviving animals appeared normal at day 1, except at the highest dose tested animals appeared normal at day 2.
Gross pathology:
For animals that died, congested lungs was the major pathological finding.

Applicant's summary and conclusion

Interpretation of results:
Category 5 based on GHS criteria
Remarks:
Not classified according to Regulation (EC) No 1272/2008
Conclusions:
LD50 = 2950 mg/kg bw