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Description of key information

The test substance inhibits CYP2A5 and CYP2A6 in an in vitro assay with liver microsomes (rat and human) (Rahnasto 2002). A strain difference in aldehydeoxidase activity in different strains of mice cannot be excluded (Al-Salmy 2001).
The cytotoxicity of the test substance towards Bluegill BF-2 cells was determined using inhibition of cell replication, protein synthesis, neutral red uptake, and colony formation as markers for cytotoxicity (Shen et al., 2000). Inhibition of cell replication proved to be the most sensitive with an ID50 of 3.773 mM. The results of the study indicate a direct relationship between the exposure concentration of the test substance and observed cytotoxic effects.
The cytotoxicity of the test substance is enhanced under influence of temperature as tested in normal HeLa cells and transformed cell lines (Ishida et al. 1983).

Additional information

The test substance inhibits CYP2A5 and CYP2A6 in an in vitro assay with liver microsomes (rat and human) (Rahnasto 2002). A strain difference in aldehydeoxidase activity in different strains of mice cannot be excluded (Al-Salmy 2001).

The cytotoxicity of the test substance towards Bluegill BF-2 cells was determined using inhibition of cell replication, protein synthesis, neutral red uptake, and colony formation as markers for cytotoxicity (Shen et al., 2000). Inhibition of cell replication proved to be the most sensitive with an ID50 of 3.773 mM. The results of the study indicate a direct relationship between the exposure concentration of the test substance and observed cytotoxic effects.

The cytotoxicity of the test substance is enhanced under influence of temperature as tested in normal HeLa cells and transformed cell lines (Ishida et al. 1983).