Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 242-060-2
CAS number: 18172-67-3
Summary of results of the chromosomal
aberration study with Turpentine oil CAS 8006-64-2
concentration in µL/mL
in % of control
Exposure period 4 hrs without S9 mix. Experiment 1
Exposure period 22 hrs without S9 mix. Experiment 2
Exposure period 4 hrs with S9 mix Experiments 1 and 2
22 hrs, expt 1
22 hrs, expt 2
cells carrying exchanges
of 200 metaphases per culture
frequency statistically significant higher than corresponding control
0.5 % (v/v)
3 CPA 15.0
4 CPA 7.5
Test item Turpentine oil CAS
8006-64-2, dissolved in THF, was assessed for its potential to induce
structural chromosomal aberrations in human lymphocytes in vitro in
two independent experiments. The following study design was performed:
Without S9 mix
With S9 mix
Exp. I & II
In each experimental group two
parallel cultures were analysed. Per culture at least 100 metaphases
were scored for structural chromosomal aberrations.
The highest applied concentration in
the pre-test on toxicity (5.0 µL/mL of the test item) was chosen with
respect to the current OECD Guideline 473.
Dose selection of the cytogenetic
experiment was performed considering the toxicity data in accordance
with OECD Guideline 473.
In Experiment I in the absence of
S9 mix, concentrations showing clear cytotoxicity were not scorable
for cytogenetic damage. In Experiment II in the absence of S9 mix,
cytotoxicity was observed at the two highest evaluated concentrations.
In Experiment I in the presence of S9 mix, no cytotoxicity was
observed up to the highest applied concentration. In Experiment II in
the presence of S9 mix the highest applied concentration was not
evaluable due to low metaphase numbers.
In both independent experiments,
neither a statistically significant nor a biologically relevant
increase in the number of cells carrying structural chromosomal
aberrations was observed after treatment with the test item.
No evidence of an increase in
polyploid metaphases was noticed after treatment with the test item as
compared to the control cultures.
Appropriate mutagens were used as
positive controls. They induced statistically significant increases (p
< 0.05) in cells with structural chromosome aberrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
På den här webbplatsen används kakor. Syftet är att optimera din upplevelse av den.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again