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EC number: 618-882-6
CAS number: 928771-01-1
potential was assessed in a GLP-compliant guideline study (method B10 of
directive 2004/73/EC). Based on results obtained from an initial
cytotoxicity screen, test concentrations of 312.5-2500 ug/ml were used
in the absence and in the presence of phenobarbitone/B-naphthaflavone
induced rat S9 fraction, and the study run using 2 independent repeats.
An oily precipitate was observed at 1250 ug/plate and above, but this
was not cytotoxic. No increase in the frequency of cells with
aberrations or the incidence of polyploidy was recorded in two
independent experiments in the absence or presence of S9 fraction, up to
the maximum dose tested (2500 ug/ml). A satisfactory response was
obtained with the positive control substances (-S9 mitomycin c; +S9
cyclophosphamide) in both experiments. The results indicate that NExBTL renewable
diesel is not clastogenic to human lymphocytes in vitro.
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