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EC number: 618-882-6
CAS number: 928771-01-1
Short-term toxicity of NExBTL (WAF) toward juvenile
rainbow trout (Oncorhynchus mykiss) was determined in GLP-compliant
study according to OECD Guidelines for Testing Chemicals (1992) no 203
"Fish, acute toxicity tests". No mortality was observed during the test.
Sub lethal effects were not detected either. LL50 > 1000 mg/l.
long-term aquatic toxicity data for fish are available. The modelled
NOELR value (long-term), using PetroTox is > 1000 mg/L
Short-term toxicity of the test material (WAF) toward a
water flea Daphnia magna was determined in GLP compliant laboratory
according to OECD Guidelines for Testing Chemicals (2002) no 202
"Daphnia sp, Acute immobilisation test". No adverse effects were
detected in the test. EL50 > 100 mg/l.
Long-term toxicity of the test material toward an aquatic invertebrate
was determined in GLP compliant laboratory according to OECD Guidelines
for Testing Chemicals (1998) no 211 "Daphnia magna, Reproduction test".
The 14 and 21 Day EL50* (immobilisation) values for parental generation
were estimated to be greater than 100 mg/l WAF. The 21 Day EL50*
(reproduction) value was estimated to be greater than 100 m/l WAF. The
LOEC was considered to be 3.2 mg/l WAF on the basis that at this loading
rate significantly (p < 0.05) fewer youngs per adult were produced when
compared to the control. The NOEC was considered to be 1 mg/l WAF on the
basis that at this loading rate there were no significant mortalities
(immobilisation) observed in the parental generation and that there were
no significant (p > 0.05) differences between the control and the 1.0
mg/l loading rate WAF test group in terms of numbers of live young
produced per adult by Day 21.
The aquatic toxicity of NExBTL renewable diesel was tested using three
aquatic organisms from different trophic levels (fish, crustancea and
algae). Due to the poor water solubility of the substance, the organisms
were exposed to a Water Accommodated Fraction (WAF) of the test
substance which is a commonly accepted procedure for regulatory studies
on poorly soluble substances. The loading rates used in these studies
were 1000 mg/l for fish and 100 mg/l for crustancea and algae. In the
experimental toxicity tests, no adverse effects were observed. In an
acute test with sediment-dwelling amphipod, the LC50 value was 1200
mg/kg dry weight (d. w.), NOEC was 373 mg/kg d. w. and LOEC was 1165
mg/kg d. w.
Short-term toxicity of NExBTL renewable diesel (WAF) toward an aquatic
algae Scenedesmus subspicatus was determined in a GLP compliant
laboratory according to OECD Guideline 201 (Alga, Growth Inhibition
Test)". There was no statistical difference (Student's t-test, p < 0.05)
in the growth between the control and the WAF test group. E*L50 > 100
Toxicity of the test material toward micro-organisms (activated sewage
sludge) was determined in a GLP compliant laboratory study according to
OECD Guideline 209 (Activated sludge, respiration inhibition test). No
effects on respiration were observed at the test concentration of 1000
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