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EC number: 618-882-6
CAS number: 928771-01-1
Ready biodegradability of the test material was determined in a GLP compliant laboratory according to OECD Guideline 301B (Ready biodegradability; CO2 Evolution Test). As a result, the test material was found readily biodegradable.
The test material, at concentration of 10 mg carbon/l, was exposed to
activated sewage sludge micro-organisms in sealed culture vessels in the
dark at approximately 21 °C for 28 days. Following the recommendations
of ISO (1995) and Handley et al (2002), the test material was adsorbed
on the granular silica gel prior to dispersion in the test medium to aid
dispersion of the poorly water soluble test material in the test medium
and to obtain more realistic results. The degradation of the test
material was assessed by the determination of carbon dioxide produced.
Control solutions with inoculum and the standard material, sodium
benzoate, together with a toxicity control were used for validation
purposes. The test material attained 82 % degradation after 28 days and
satisfied the 10-Day window validation criterion, whereby 60 %
degradation must be attained within 10 days of the degradation exceeding
10 %. The test material can therefore be considered to be readily
biodegradable under the strict terms and conditions of OECD Guideline No
301B. REFERENCES: Handley JW, Mead C, Rausina GA, Waid LJ, Gee JC and
Herron SJ, 2002, The use of inert carriers in regulatory biodegradation
tests of low density poorly water-soluble substances, Chemosphere 48(5),
529-534. International Standards Organisation (ISO), ISO 10634, 1995,
Water Quality – Guidance for the preparation and treatment of poorly
water-soluble organic compounds for subsequent evaluation of their
biodegradability in an aqueous medium.
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