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EC number: 306-082-7
CAS number: 95912-86-0
Table 1: Reproduction (given as mean number of juveniles ±
standard deviation and as % of the control) in the treated test vessels.
Concentration [mg test item/kg soil (dw)]
Number of juveniles per treatment [mean ± SD]
Number of juveniles [% of solvent control]
333.5 ± 41.0
335.0 ± 62.5
376.5 ± 32.4
335.3 ± 38.0
317.3 ± 38.2
300.5 ± 65.9
262.3 ± 90.7
* significantly different to control (Williams t-test; 1-sided, p
Table 2: Average live weight (mg) per surviving adult worm at the
beginning of the test and after 28 d (n=80 for solvent control; n=40 for
concentrations and additional water control). Data are given as absolute
value and in percent of the initial weight (biomass).
Biomass [mg fresh weight]
Percent of the initial weight
NOEC (56 d) 500 mg/kg soil dw based on reproduction of Eisenia fetida (OECD
One study is available investigating the long-term effects of
Fatty acids, C8-C10, C12-18-alkyl esters (CAS 95912-86-0) to the
earthworm Eisenia fetida (Scheffczyk, 2013). The study was
performed according to OECD 222 (GLP) for 56 d with artificial soil.
Test concentrations up to nominal 1000 mg/kg dw were tested for impacts
on mortality, biomass and reproduction. Mortality and biomass were
investigated after 28 d whereas the number of juveniles was measured
after 56 d. No mortality and no effects on biomass were recorded after
28 d. However, effects on reproduction expressed in the number of
juveniles was observed at the highest test concentration of 1000 mg/kg
soil dw. Thus, a NOEC (56 d) of 500 mg/kg soil dw was derived. Analyzing
the results in detail shows that the number of juveniles was very
variable within the replicates at the highest concentration. In two
replicates out of four the number of juveniles were 186 and 189,
respectively whereas in the other two replicates 303 and 371 juveniles
were recorded which is in the range of the solvent control.
Nevertheless, since no indications are given in the report why the
replicates show such a high variability at the highest test
concentration the reported NOEC of 500 mg/kg soil dw has to be used for
the assessment as a worst case assumption.
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