2,2'-oxydiethanol

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Chemical burns of the rabbit cornea.

Author:

Carpenter CP, Smyth HF

Year:

1946

Bibliographic source:

Am J Ophthal 29: 1363-1372

Materials and methods

Principles of method if other than guideline:

Estimation of eye hazard within a range-finding test.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-oxydiethanol

EC Number:

203-872-2

EC Name:

2,2'-oxydiethanol

Cas Number:

111-46-6

Molecular formula:

C4H10O3

IUPAC Name:

2-(2-hydroxyethoxy)ethan-1-ol

Test animals / tissue source

Species:

rabbit

Strain:

not specified

Details on test animals or tissues and environmental conditions:

no data

Test system

Vehicle:

unchanged (no vehicle)

Controls:

yes, concurrent no treatment

Amount / concentration applied:

0.5 mL undiluted diethylene glycol

Duration of treatment / exposure:

24 hour(s)

Observation period (in vivo):

no data

Number of animals or in vitro replicates:

5

Details on study design:

The individual numerical scores of each eye treated were added together and then divided by the number of treated eyes (usually 5 animals) to obtain the score of the injury caused by the treatment. The level of 5.0 was selected by the authors of the study as representative of severe injury.
When a test is to be performed, normal albino rabbits are selected on the basis of absence of grossly visible staining of the eye by a 5% aqueous solution of fluorescein sodium, flushed with distilled water 20 seconds after application. After a two-hour interval to allow the eye to return normal, the undiluted material is applied to the centre of the cornea while the lids are retracted. Eighteen to 24 hours later, the eye is examined in strong diffuse daylight, and then stained with fluorescein, and the injury scored. Guided by the result and the table of injury grades, additional applications are made until the chemical can be assigned to one of the grades they recognize.

Results and discussion

In vivo

Results

Irritation parameter:

other: ocular irritation

Basis:

mean

Remarks:

all animals

Time point:

24 h

Score:

1

Max. score:

10

Reversibility:

not specified

Remarks on result:

other: 0.5 mL 0.5 mL undiluted gives injury of 0 to 1.0 points

Remarks:

Grade 1

Irritant / corrosive response data:

No occular irritation was noticed after application of 0.5 mL of undiluted test item.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met