Acetic acid

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

4-18 April 1995

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: The study report has not been received and therefore cannot be reviewed and evaluated in full.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

Principles of method if other than guideline:

No data reported.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

TEM 123 (CEL 350 20 H SL)

IUPAC Name:

TEM 123 (CEL 350 20 H SL)

Details on test material:

10% acetic acid. Liquid. Water soluble concentrate (ready for use). Batch number: 0395. Substance was stable under test conditions.

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No data reported.

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

Assessment of toxicity effects to earthworms were carried out on days 7 and 14.

Test substrate

Vehicle:

no

Details on preparation and application of test substrate:

Artificial soil was prepared using: 10% Sphagnum peat, 20% Kaolin clay, 69% industiral sand, 1% calcium, deionised water (to water content of 25%).

Test organisms

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

Species: Eisenia foetida (Savigny) and Eisenia fetida andrei (Bouche) - 1826 (Earthworms). At test initiation: adult worms, 7 months old. Weight at test initiation: 3378-3660 mg/ 10 earthworms. Source: worms were taken from a healthy stock within the test facility, which were kept under laboratory conditions. Acclimitisation: 24 ours in moist artificial soil.

Study design

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Post exposure observation period:

No data reported.

Test conditions

Test temperature:

20 +/- 2˚C

pH:

No data reported.

Moisture:

25%

Details on test conditions:

Tests were carried out in 1L glass vessels with lids in a climate controlled chamber. The test chambers were continually exposed to light at 400-800 lux (daylight). Number of worms per vessel: 10. Number of replicates: 4.

Nominal and measured concentrations:

Nominal concentrations: 0, 2.86, 28.6, 71.4, 143, 286 mL/kg dry weight. These nominal concentrations correspond to field doses of: 0, 2000, 20000, 50000, 100000, 200000 L/ha.

Reference substance (positive control):

yes

Remarks:

100% 2-chloracetamide

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

28.6 other: mL/kg d.w.

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 2914 mg as/kg d.w.

Effect concentrations 2

Duration:

14 d

Dose descriptor:

LOEC

Effect conc.:

71.4 other: mL/kg d.w.

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 7286 mg as/kg d.w.

Effect concentrations 3

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

71.4 - 143 other: mL/kg d.w.

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 7286-14571 mg as/kg d.w.

Details on results:

The test substance was found not to have had a negative effect on the body weight of surviving eatrhworms. There was a statistically significant increase in body weight of the surviving earthworms at the 28.6 mL/ kg d.w. concentration.

Results with reference substance (positive control):

No data reported.

Reported statistics and error estimates:

No data reported.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 14-day LC50 for earthworms of TEM 123is 71.4-143 mL/kg of dry artificial soil based on nominal concentrations. This converts to 7286-14571 mg as/kg of dry artifical soil.

Executive summary:

Lang (1995) has been given a Klimisch score of 4 - not assignable (Klimisch et al, 1996) as the study has not been received and therefore has not been properly evaluated. A summary of the study is included in the EU Draft Assessment Report for Acetic Acid (August 2008).