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EC number: 931-597-4
CAS number: -
Irritation score and irritation score mean
In the negative control, 4/4 eggs showed no hyperemia, no hemorrhage and
no coagulation at the end of the 5-minute observed period. In the
positive control, 4/4 eggs showed an hemorrhage within the first 30
seconds and coagulation before the end of the first 2 minutes of
observation. For instance, the negative and positive controls induced a
response that falls within the classification of non-irritating
(ISmean=0) and severely irritating (ISmean=14) respectively. For this
reason, the test is considered valid.
All eggs treated with test item MIXED ASH showed haemorrhage and
coagulation between 30 and 120 seconds of observation period. Mean
irritation score calculated after treatment with test item MIXED ASH was
12. The results are summarised in the Table 4.
Table 4 Mean irritation score and irritation category
Individual and mean results are presented in Table 5 and 6.
Table 5 Irritation Score Mean (mean values)
No statistical analysis. IS: Irritation score calculated for each eggs
as follows: IS = score obtained for hyperemia + score obtained for
haemorrhage + score obtained for coagulation
Table 6 Appearance time of hyperemia, hemorrhage and coagulation.
Results are expressed in second (s)
Eye irritation properties of Ash were determined in a GLP compliant
laboratory with an in vitro Hen's Egg Test Chorioallantoic Membrane
(HET-CAM) test. The test method used is based on the general
requirements of French Official Text, Annex IV, dated on December 26th,
1996, Invitox protocol No. 47 dated on January 1992 and NIH publication
No. 06-4515 (March 2006). After 10-day incubation of fertilised eggs,
0.3 ml of pulverised Ash was placed onto the CAM for 20 second. The CAMs
were observed for a period of 5 min by using candling light. The
observed reactions were haemorrhage and coagulation of the CAM. Thus,
Ash is severe irritant for the eye.
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