Registration Dossier

Administrative data

Endpoint:
neurotoxicity
Remarks:
other: in vitro
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Histopathologic approaches to chemical toxicity using primary cultures of dissociated neural cells grown in chamber slides.
Author:
Bolon B, Dorman DC, Bonnefol MS, Randall HW, and Morgan KT
Year:
1993
Bibliographic source:
Toxicologic Pathology 21, 465-479.

Materials and methods

GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium formate
EC Number:
205-488-0
EC Name:
Sodium formate
Cas Number:
141-53-7
Molecular formula:
CHO2Na
IUPAC Name:
sodium formate
Details on test material:
Sodium formate.
Specific details on test material used for the study:
Sodium formate.

Test animals

Species:
mouse
Strain:
CD-1

Results and discussion

Any other information on results incl. tables

Neurotoxic effects seen with sodium formate after 8 h of  incubation:

1) Toxicity in developing cultures: 
Sodium formate: toxic  at </= 40 mM. 
Toxicity of model toxicants:
Cytosine arabinoside: gliotoxic at 0.01 mM.

2) Mature cultures
Sodium formate:  neuronotoxic at </= 60 mM. 
                 cytotoxic  at >/= 120 mM.

Toxicity of model toxicants:
Glutamate neuronotoxic  at </= 5.0 mM; 
Methyl iodide neuronotoxic  at </= 2.50 mM 
               cytotoxic  at >/= 5.0 mM.

Sodium formate caused lesions mainly in large, polygonal neurons.

Applicant's summary and conclusion

Conclusions:
Neuronotoxicity in vitro seen at >/= 40 mM sodium formate