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EC number: 202-615-1 | CAS number: 97-88-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Butyl methacrylate
- EC Number:
- 202-615-1
- EC Name:
- Butyl methacrylate
- Cas Number:
- 97-88-1
- Molecular formula:
- C8H14O2
- IUPAC Name:
- butyl methacrylate
- Details on test material:
- Supplier: Mitsubishi Gas Chem. Co., Inc.
Batch No.: NG60912
Purity: 99.6 %
Storage Conditions: Cool and dark location
Constituent 1
Method
- Target gene:
- Histidine operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9: Rat liver, induced with phenobarbital and 5,6-benzoflavone
- Test concentrations with justification for top dose:
- -S9 mix: 9.77, 19.5, 39.1, 78.1, 156, 313 and 625 µg/plate
+S9 mix: 9.77, 19.5, 39.1, 78.1, 156, 313, 625 and 1250 µg/plate - Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: -S9 mix; 2-(2-Furyl)-3-(5-nitro-2-furyl) acrylamide (TA100, TA98, WP2 uvrA), Sodium azide (TA1535), 9-Aminoacridine (TA1537) +S9 mix; 2-Aminoanthracene(all strains)
- Details on test system and experimental conditions:
- 100μl of the solvent used, the test substance solution and the positive control substance was placed in a capped tube, and then 500μl of 0.1M sodium phosphate buffer (pH7.4) for the direct method, or 500μl of the S9 mix for the metabolic activation method was added. Next, after adding 100μl of the pre-incubated test bacteria strain suspension, this was subject to 20 minutes of shaking incubation (pre-incubation) at 37°C using an incubator shaker. After incubation was completed, 2ml of top agar was added and the contents mixed. Then, the mixed solution was poured and evenly spread on the plate. After the layered top agar solidified, each plate was sealed with cellophane tape and incubated for 48 hours under conditions of 37°C using an incubator.
Three plates per dose were used. Also, to verify reproducibility, these tests were independently performed two times. - Evaluation criteria:
- The number of reverse mutated colonies increased to nearly double that of the control solvent, and when the reproducibility and the test substance dose dependence was confirmed, it was deemed positive
- Statistics:
- A statistical analysis was not performed
Results and discussion
Test results
- Species / strain:
- other: Salmonella typhimurium TA100, TA1535, TA98, TA1537, Escherichia coli WP2 uvrA
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- >= 156 µg/plate in the 5 strains without S9. >=313 µg/plate (TA100, Ta1535, TA98, TA1537) and >= 625 µg/plate (WP2 uvrA) with S9
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- This chemical was not mutagenic in Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvrA, with or without an exogenous metabolic activation system.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
In a valid guideline study the test substance was not mutagenic in Salmonella typhimurium TA100, TA1535, TA98, TA1537 and Escherichia coli WP2 uvrA, with or without an exogenous metabolic activation system. - Executive summary:
The potential of n-butyl methacrylate to induce reverse mutation in Salmonella typhimurium (strains: TA 1535, TA 1537, TA 98, and TA 100) and in Escherichia coli WP2 uvrA was evaluated in accordance with the international guidelines (OECD 471, Commission Directive No. B13/14) in compliance with the Principles of Good Laboratory Practice.
n-Butyl methacrylate was tested in two independent experiments, with and without a metabolic activation system, both performed according to preincubation method. Bacterias were exposed to the test item at 7 or 8 dose-levels (three plates/dose-level) selected from a preliminary toxicity test: 9.77, 19.5, 39.1, 78.1, 156, 313 and 625 µg/plate without S9 and 9.77, 19.5, 39.1, 78.1, 156, 313, 625 and 1250 µg/plate with S9. After 48 to 72 hours of incubation at 37°C, the revertant colonies were scored.
n-butyl methacrylate did not induce any noteworthy increase in the number of revertants, both with and without S9 mix, in any of the four Salmonella typhimurium strains and in Escherichia coli WP2 uvrA.
Under these experimental conditions, n-butyl methacrylate did not show any mutagenic activity in the bacterial reverse mutation test with Salmonella typhimurium and Escherichia coli.
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