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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to soil microorganisms

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Administrative data

Link to relevant study record(s)

Description of key information

See below.

Key value for chemical safety assessment

Long-term EC10 or NOEC for soil microorganisms:
100 mg/kg soil dw

Additional information

Iso E Super was tested for its effect on the activity of soil microflora (nitrogen transformation) according to OECD TG 216 Nitrogen Transformation Test. Nitrogen-transformation in soil enriched with lucerne meal was determined. Test item treated soil was compared with a non-treated soil. NO3 -nitrogen formed in the nitrification process was determined by continuous flow analysis. The following test rates of Iso E Super were used: 3.16, 10, 31.6, 100 and 316 mg test item/kg soil dw. Sodium chloride was used as reference item. Moisture content was 47% to 48% of WHCmax and temperature was 20°C ± 2°C in the dark. No adverse (deviations to control below 25%) of the test item on nitrate content in soil were observed at day 28 up to a concentration of 100 mg test item/kg soil dw. The soil nitrate formation rates per day did not exceed the trigger range of ± 25% from the 0 - 28 day interval up to a concentration of 100 mg test item/kg soil dw. In conclusion, Iso E Super had no impact on nitrogen transformation (nitrate content and nitrate formation rate) of soil microorganisms when applied up to 100 mg test item/kg soil dw treatment. An application of 316 mg test item/kg soil dw caused a significant increase above the trigger value for nitrate content and nitrate rate. The NOEC appears to be 100 mg test item/kg soil dw.