Glyoxylic acid

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study was performed prior to the implementation of GLP and OECD Guidelines, but is in compliance with the principles described in OECD Guideline 401.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Glyoxylic acid

EC Number:

206-058-5

EC Name:

Glyoxylic acid

Cas Number:

298-12-4

Molecular formula:

C2H2O3

IUPAC Name:

2-oxoacetic acid

Details on test material:

Glyoxylic Acid, 50 %

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: own breeding
- Age at study initiation: young adult
- Weight at study initiation: 80-96 g
- Fasting period before study: 16 hours
- Housing: plastic cages
- Diet: standard diet ALTROMIN 1324, Altrogge Lage/Lippe, Germany ; ad libitum
- Water: tap water, ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Doses:

1250, 2000, 3200, 5000 mg/kg bw

No. of animals per sex per dose:

10 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: body weights were recorded one a week
- Necropsy of survivors performed: yes

Statistics:

Statistics were performed using the inverse cumulative distribution function (ProbitFunction according to LINDER and WEBER).
Confidential intervalls were claculated according to the merhod of CAVALLI-SFORZA.

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

2 528 mg/kg bw

Based on:

test mat.

Mortality:

1250 mg/kg bw 0/10 ;
2000 mg/kg bw 2/10 ;
3200 mg/kg bw 8/10 ;
5000 mg/kg bw 10/10

Clinical signs:

other: Animals that died showed a ragged breathing, disturbance of equilibrium and cramps and were convulsing in ventral position.

Gross pathology:

In animals that died a pale, white coloured small intestine was noted.
Necropsy revealed no abnormalities in animals that survived.

Applicant's summary and conclusion

Executive summary:

In this acute oral toxicity study (Hoechst AG, Pharma Forschung Toxikologie, 1975), groups of fasted, young adult Wistar rats (10/dose) were given a single oral dose of Glyoxylic Acid 50 % in water at doses of 1250, 2000, 3200 or 5000 mg/kg bw and observed for 14 days. In animals that died a pale, white coloured small intestine was noted. Necropsy in animals that survived revealed no abnormalities. The oral LD50 value was calculated to be 2528 mg/kg bw.