Glyoxylic acid

Biodegradation in soil

Administrative data

Endpoint:

biodegradation in soil: simulation testing

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

the study does not need to be conducted because the substance is readily biodegradable

Justification for type of information:

The mean biodegradation of Glyoxylic Acid 50% based on DOC decrease was found to be 98% at the end of the 14d test. In accordance with Regulation 1907/2006/EC, Annex IX, 9.2.1.2 column 2, testing does not need to be conducted as Glyoxylic Acid 50% is readily biodegradable.

Cross-reference

Reason / purpose for cross-reference:

data waiving: supporting information

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 18. May to 02. June 2009

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Harlan Laboratories Ltd., Quality Assurance GLP, Zelgliweg 1, 4452 Itingen, Switzerland

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of activated sludge:
wastewater treatment plant (ARA Ergolz II, Füllinsdorf / Switzerland) treating predominantly domestic wastewater
- Storage conditions:
room temperature
- Pretreatment:
The sludge was washed twice with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of
the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated
- Concentration of sludge:
30 mg dry material per liter

Duration of test (contact time):

14 d

Initial conc.:

30.4 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Reference substance:

benzoic acid, sodium salt

Parameter:

% degradation (DOC removal)

Value:

98

St. dev.:

2.8

Sampling time:

3 d

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Due to the fact that > 98 % of Glyoxylic Acid 50 % were degraded within the 10-d window of the incubation period, the test item can be classified as readily biodegradable under the conditions of this study.

Executive summary:

In a Ready Biodegradability study (OECD 301 A) Glyoxylic Acid 50% was assessed over a 14 day period by the DOC die away test. The nominal test concentration was 30.4 mg/L. The test material attained > 98 % biodegradation after 3 and 10 days, respectively. The reference compound sodium benzoate showed a mean biodegradability of > 90 % within 7 days, thus confirming suitability of the activated sludge.

These results indicate that Glyoxylic Acid 50% is readily biodegradable under conditions of the test.