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EC number: 252-104-2
CAS number: 34590-94-8
DOWANOL 50B, the methyl ether of dipropylene
glycol, a colorless liquid was evaluated for chronic skin absorption in
Male rabbits were selected and divided into
groups of at least five animals each. Dipropylene glycol methyl ether
was applied over the abdominal skin using the following technique. A pad
of absorbent cotton about 3”×3” in size and sufficiently thick just to
absorb the volume of the test material was applied to the clipped
abdomen of the rabbit. The proper dose of the compound was added to the
cotton and the pad was then covered with an impervious saran film about
5”×5”. This saran film was covered with a heavy cloth, and the whole
application was then strapped onto the animal with adhesive tape.
Dipropylene glycol methyl ether was thus applied five times a week over
a period of three months at four dosage levels: 1.0, 3.0, 5.0 and 10.0
ml/kg. A separate group of five animals to which distilled water was
bandaged served as controls.
The rabbits received the stock diet of
commercial rabbit chow and water ad libitum. The rabbits were weighed
before the application of each daily dose of the test compound. Control
blood counts were taken before the start of the study and on the
thirtieth and ninetieth day of the application of the compound. Possible
significance between means was studied by students test of “t”. On the
ninetieth day the rabbits were autopsied and tissues taken from the
liver, kidneys, spleen, adrenal, heart, lung and occasionally stomach,
for histopathological examination. Such sections were stained with
haematoxylin and eosin.
Dipropylene glycol methyl ether at the 10
ml/kg and 5 ml/kg produced some narcosis. However narcosis was not
observed at lower dose levels (1.0 and 3.0 ml/kg). Mortality was high at
the 10.0 ml/kg dosage level, slight at 5.0 ml/kg and absent at the 1.0
and 3.0 ml/kg dose levels.
No adverse body weight changes occurred at
any level except just prior to death in those animals that succumbed,
presumably to the narcotic effects of the top dosage levels.
No haematological changes occurred at any
dosage level. No significant organ weight changes occurred at any dosage
level. Observations for gross pathology revealed only gastric distension
and occasional gastric irritation in those animals dying at the 10 ml/kg
Histopathological studies conducted on the
liver, lung, spleen, adrenal, heart, testes and stomach of those animals
receiving the 5.0 and 10.0 ml/kg dosage levels revealed no changes. The
kidneys of those animals on the 10.0 ml/kg level showed some granular
and some hydropic changes, at the 5.0 ml/kg same kidney abnormalities
were observed but they were of no greater intensity than those observed
in some of the controls.
The effect of severe (repeated and
prolonged) exposure to the skin was slight, being similar to that caused
by distilled water under similar conditions.
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