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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
08. Mar 1965 - 21. Mar 1965
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1966
Report date:
1966

Materials and methods

Principles of method if other than guideline:
BASF-Test: The study was conducted according to an internal BASF method which in principle is comparable to the OECD Guideline 401.
A test group consisting of 5 animals/sex was treated by single gavage application with an aqueous solution of the test substance. The animals were observed for mortality and for clinical symptoms of toxicity. They were weighed prior treatment and thereafter, day 3, day 7 and day 13 post-treatment. At the end of the observation period of 14 days, the surviving animals were sacrificed for the purpose of necropsy; animals that died during the observations period also were subjected to necropsy.
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
1,1',1''-nitrilotripropan-2-ol
EC Number:
204-528-4
EC Name:
1,1',1''-nitrilotripropan-2-ol
Cas Number:
122-20-3
Molecular formula:
C9H21NO3
IUPAC Name:
1,1',1''-nitrilotripropan-2-ol
Test material form:
solid
Specific details on test material used for the study:
- Name of test material (as cited in study report): Triisopropanolamin
- Analytical purity: ca. 92-95 %
- Impurities (identity and concentrations): ca. 3-6 % Diisopropanolamine; <1 % Monoisopropanolamine

Test animals

Species:
rat
Strain:
other: US-rats
Sex:
male/female
Details on test animals or test system and environmental conditions:
- Weight at study initiation: male: 211 g (mean), female: 171 g (mean)

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
water
Doses:
200, 1600, 3200, 4000, 5000, 6400 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 7 days
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 4 000 mg/kg bw
Based on:
test mat.
Mortality:
- 8 out of 10 animals died exposed to 6400 mg/kg bw
- 8 out of 10 animals died exposed to 5000 mg/kg bw
- 6 out of 10 animals died exposed to 4000 mg/kg bw
- 0 out of 10 animals died exposed to 3200, 1600, or 200 mg/kg bw
Clinical signs:
- 6400, 5000, 4000 mg/kg bw: After the application piloerrection, high stepping gait, slight stagger, mouth discharge. One hour after application beginning eye discharge, diarrhea, lateral and abdominal position, slight tremor, partly dyspnoea. On the following day heavy stagger, eye and nose crusts, hind limbs seemed to be cramped. Ruffled fur smeared at anogenital region, nose and eyes. Surviving animals were very timid and recovered from day 4.
- 3200, 1600 mg/kg bw: Symptoms similar but less distinctive as in higher doses.
- 200 mg/kg bw: On the day of application piloerrection, chewing motions, high stepping gait. On the next 2-3 days animals showed crusted noses, irregular respiration and heavy ruffled fur.
Body weight:
no data
Gross pathology:
- 6400 mg/kg bw: 1x gastro-intestinal irritation, 1x remaining substance in stomach
- 3200 mg/kg bw: 1x bronchitis and bronchiectasia
- 1600 mg/kg bw: 1x bronchitis and bronchiectasia

Any other information on results incl. tables

Mortality:

Dose (mg/kg bw)  Conc. (%)  1 h  24 h  48 h  7 days
6400  30  1/10  7/10  8/10  8/10  
5000  30  0/10  8/10  8/10  8/10  
4000  30  0/10  6/10  6/10  6/10  

3200

 30  0/10  0/10  0/10  0/10  
1600  20  0/10  0/10  0/10  0/10  
200  2  0/10  0/10  0/10  0/10  

Applicant's summary and conclusion