Lanthanum chloride, anhydrous

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Guidelines of the Department of Transportation (D.O.T.), Code of Federal Regulations, Title 49, Part 173

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): Rare Earth Solution Code #1455
- Molecular formula (if other than submission substance): LaCl3*3(H2O)
- Physical state: amber liquid
- Concentration of solution: 100%
- Analytical purity: no data

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Taconic Farms Inc.
- Weight at study initiation: 100 - 150 g
- Fasting period before study: overnight
- Housing: 5 per wire mesh suspended cage
- Diet (Purina Laboratory Chow) and water ad libitum
- Acclimation period: 7 d

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

MAXIMUM DOSE VOLUME APPLIED: 0.9 mL

RANGE FINDING STUDY
- Concentrations tested: 3980, 6320, and 10000 mg/kg
- 2 animals per concentration
- result: No. dead/No. dosed: 10000 mg/kg: 2/2; 6320 mg/kg: 2/2; 3980 mg/kg: 1/2

Doses:

2820, 3550 and 4470 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 48 hours
- Frequency of observations and weighing: clinical signs were noted immediately after dosing and at 4, 24, and 48 hours; body weights were recorded at dosing and at 48 hours for the survivors.
- Animals found dead or terminated after 48 hours were subjected to a macroscopic examination of the viscera.

Statistics:

LD50 was calculated according to the method of: Litchfield and Wilcoxon, Pharmacol. and Exp. Ther. 96(99): 1499

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

3 200 mg/kg bw

95% CL:

> 2 883 - < 3 552

Remarks on result:

other: 48 hours; Test substance: Rare Earth Solution Code #1455

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

2 621 mg/kg bw

95% CL:

> 2 361 - < 2 909

Remarks on result:

other: 48 hours; Test substance: Lanthanum chloride, anhydrous

Mortality:

4470 mg/kg: 8 out of 10 died within 24 h, 1 out of 10 died within 48 h
3550 mg/kg: 6 out of 10 died within 24 h, 2 out of 10 died within 48 h
2820 mg/kg: 2 out of 10 died within 24 h, no further deaths within 48 h

Clinical signs:

other: 4470 mg/kg: all animals were prostate after dosing and at 4 h. 3550 mg/kg: all animals were lethargic after dosing and didn´t recover until they died or were sacrificed. 2820 mg/kg: all animals were lethargic after dosing and at 4 h; after 24 h 2 animals

Gross pathology:

4470 mg/kg: dead animals: test solution in stomach and intestine; sacrificed animal: no gross pathology - Diarrhea
3550 mg/kg: dead animals: test solution in stomach and intestine; sacrificed animals: no gross pathology - Diarrhea
2820 mg/kg: dead animals: test solution in stomach and intestine; sacrificed animals: no gross pathology (3 animals) or distended stomachs (5 animals)
No gross pathology was performed with surviving animals.

Any other information on results incl. tables

Mortality:

Concentration (mg/kg)	Mortality: No. Dead / No. Dosed
	4 h	24 h	48 h
males
4480	0/5	4/5	1/5
3550	0/5	4/5	0/5
2820	0/5	2/5	0/5
females
4480	0/5	4/5	0/5
3550	0/5	2/5	2/5
2820	0/5	0/5	0/5