Exo-1,7,7-trimethylbicyclo[2.2.1]hept-2-yl acrylate

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012-09-26 to 2012-10-17

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

OECD 211, GLP, analytical monitoring.

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Version / remarks:

(2008)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: STRAUS (Clone 5)
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), Germany
- Age at study initiation (mean and range, SD): 2 to 24 hours
- Length at study initiation (length definition, mean, range and SD): no data
- Weight at study initiation (mean and range, SD): mean weight: no data
- Method of breeding: Laboratory bred
- Food type: unicellular green algae, Pseudokirchneriella subcapitata and , Desmodesmus subspicatus
- Amount: corresponding to 0.2 mg Carbon per daphnia and day
- Frequency: daily

ACCLIMATION
- Acclimation period: test organism is bred at test facility
- Health during acclimation (any mortality observed): no data

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

Total hardness: test concentrations: 157 to 180 mg/L as CaCO3

Test temperature:

18 - 22 °C ± 2 °C constant

pH:

test concentrations: 7.23 to 7.82
control: 8.18 to 8.23

Dissolved oxygen:

test concentrations: 7.56 to 8.52 mg/L
control: 8.30 to 8.40 mg/L

Nominal and measured concentrations:

see table below

Details on test conditions:

TEST SYSTEM
- Test vessel: sealed glass flasks
- Type (delete if not applicable): closed because of volatile test substance
- Material, size, headspace, fill volume: 4.5 (ID) x 9.5 (H) cm, ca. 130 mL; filled up with the test solutions having nearly no headspace to reduce losses of the test item
- Aeration: no aeration
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4, according to Elendt (1990), modified to a total hardness of 160 to 180 mg CaCO3/L
- Total organic carbon: 160 to 180 mg CaCO3/L
- Culture medium different from test medium: no
- Intervals of water quality measurement: Dissolved oxygen concentration, pH-values, total hardness and water temperature were measured once
every 7 days, in fresh and old media, in one replicate of the control and the saturated solution or the next appropriate dilution level.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hr light / 8 hr dark cycle
- Light intensity: max. 20 µE*mE-2*sE-1

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

Mortality of parental daphnids
The number of parental daphnids which were immobilized or dying during the test and day of death were observed and recorded once a day at
least at the same time as the offspring was counted. Dead specimen were removed.

Neonates
First appearance of juveniles was checked daily. The number of neonates (alive and dead progeny) was counted and dates of release were recordedonce a day from the first day of production of neonates unti! the end of the test. The neonates (alive and dead) were removed after counting and
before addition of algae to prevent from consuming food intended for the adults. The number of aborted eggs or dead offspring and dates of
observation were recorded.

Condition of parental daphnids
Abnormalities (e.g. swimming behaviour, number of male and winter eggs) were observed and recorded at each day of observation.

Total length and dry body weight of parental daphnids
At the end of the test total length of each daphnid and the mean dry body weight of the daphnids alive in all dilution levels and control were
determined.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

0.524 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: calculated by sigmoidal dose-response regression; EC50: 0.277 - 0.838 mg/L (Confidence interval, p=95 %)

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

0.092 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

0.277 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

0.874 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks:

adult daphnids

Remarks on result:

other: calculated by sigmoidal dose-response regression; EC50: 0.277 - 2.94 mg/L (Confidence interval, p=95 %)

Details on results:

The test item induced biologically significant adult mortality in concentration levels of 0.838 mg/L (30%), 2.94 mg/L (100 %) and 8.89 mg/L (100%) after 21 days. In the concentration levels 0.0920 and 0.277 mg/L and in the control all parental daphnids survived till the end of the test after 21 days. The EC10-value for the adult mortality was calculated by sigmoidal dose-response regression to be 0.760 mg/L (95 % confidence limits: 0.277 – 2.94 mg/L). The EC50-value was calculated accordingly to be 0.900 mg/L (95 % confidence limits: 0.277 – 2.94 mg/L).
The mean body length of the surviving parental daphnids in the concentration levels of 0.0920 to 0.838 mg/L were in the range of 4.86 to 5.50 mm per daphnid and 5.50 mm per daphnid in the control group. The mean dry body weight was in the range of 0.900 to 1.26 mg per daphnid in the concentration levels of 0.0920 to 0.838 mg/L and 1.15 mg per daphnid in the control.

Results with reference substance (positive control):

- Results with reference substance valid?: yes
- Relevant effect levels: EC50: 1.44 mg/L (95% confidence limits: 1.29 - 1.52 mg/L) , immobilization after 24 hr.

Reported statistics and error estimates:

One Way Analysis of Variance on Ranks, Dunnett's method, p=0.05

Measured concentrations of Isobornyl acrylate in the non GLP preliminary Test

Sampling date	2012-09-18 0 h (Fresh media)		2012-09-19 24 h (Old media)		2012-09-19 24 h (Fresh Media)		2012-09-20 48 h (Old media)
Nominal test item concentration [mg/L]	Isobornylacrylate
	Meas. conc. [mg/L]	%	Meas. conc. [mg/L]	%	Meas. conc. [mg/L]	%	Meas. conc. [mg/L]	%
20.0	15.9	80	15.3	77	Not analysed due to 100 % mortality
2.00	1.78	89	1.62	81	1.68	84	1.63	82
0.200	0.184	92	0.163	82	0.196	98	0.170	85
Control	< LOQM		< LOQM		< LOQM		< LOQM

Meas. conc.= Measured concentration, single injection, dilution factor taken into account

% = Percent of the nominal concentration of the test item

LOQ_M= Limit of quantification of theanalytical method(10 µg/L of the test item)

Validity criteria fulfilled:

yes

Remarks:

To prove the validity of the test system and test conditions, an acute immobilization test according to DIN 38412 L 11 is carried out with potassium dichromate as reference item once per month.

Conclusions:

In this valid guideline study (OECD 211, semi-static conditions), the 21 day reproductive toxicity to daphnids was determined for Isobornyl acrylate. The EC50 for Daphnia magna was 0.524 (0.277 - 0.838) mg/L, the NOEC: 0.092 mg/L and the LOEC: 0.277 mg/L. All effect values given are based on the geometric mean measured test concentrations.

Executive summary:

 The 21-day-chronic toxicity (OECD 211) of Isobornyl acrylate to Daphnia magna STRAUS (Clone 5) was studied under semi-static (daily renewal) conditions. Daphnids were exposed to control, and test chemical at measured concentrations of 0.0920 - 0.277 - 0.838 - 2.94 - 8.89 mg/L, which corresponds to mean recovery rates of 84 to 92 % of the nominal values.T

 

The reduction of the reproductive output was the most sensitive effect in this study

The 21 day EC50 based on reproductive effects was 0.524 (CI 95%: 0.277 - 0.838) mg a.i./L. The 21-day NOEC based on reproductive effects was 0.092 mg a.i./L (the highest test concentration with no significant reduction of the reproductive output). The Lowest Observed Effect Concentration (LOECreproduction) was assessed at 0.277 mg/L.

 

The average number of living juveniles per parent alive at the end of the test after 21 days was 97 in the control group. A statistically significant reduction of the reproductive output alive in comparison to the control was determined at the concentration levels of 0.277 and 0.838 mg/L (One Way Analysis of Variance, Dunnett’s method, p = 0.059). At the concentration levels of 2.94 and 8.89 mg/L, no juveniles occurred due to 100 % mortality of the daphnids. 

The coefficient of variation of the number of living offspring produced per parent was 10 % in the control.In the concentration levels 0.0920 and 0.277 mg/L the coefficient of variation was 9 and 19 %. A coefficient of variation of more than 25 % is regarded to be biologically significant. The coefficient of variation of 64 % in the concentration level of 0.838 mg/L is biologically significant.

 

No stillborn juveniles and one aborted egg were produced by the control group during the exposure period of 21 days. Related to the total number of produced juveniles (dead + alive), the fraction of dead juveniles (sum of stillborn juveniles and aborted eggs) was 5 % in the concentration level of 0.0920 mg/L, 10 % in the concentration level of 0.277 mg/L and 67 % in the concentration level of 0.838 mg/L during the exposure period of 21 days

 

The test item induced biologically significant adult mortality in concentration levels of 0.838 mg/L (30%), 2.94 mg/L (100 %) and 8.89 mg/L (100%) after 21 days. In the concentration levels 0.0920 and 0.277 mg/L and in the control all parental daphnids survived till the end of the test after 21 days.
The EC10-value for the adult mortality was calculated by sigmoidal dose-response regression to be 0.760 mg/L (95 % confidence limits: 0.277 – 2.94 mg/L). The EC50-value was calculated accordingly to be 0.900 mg/L (95 % confidence limits: 0.277 – 2.94 mg/L

 

The mean body length of the surviving parental daphnids in the concentration levels of 0.0920 to 0.838 mg/L were in the range of 4.86 to 5.50 mm per daphnid and 5.50 mm per daphnid in the control group.The mean dry body weight was in the range of 0.900 to 1.26 mg per daphnid in the concentration levels of 0.0920 to 0.838 mg/L and 1.15 mg per daphnid in the control.

 

The validity of the test system was proven with potassium dichromate as reference item.

 

 

Results Synopsis 

Test Organism: Daphnia magna

Test Type: semi-static, daily renewal, closed system

 

NOEC(reproduction): 0.092 mg a.i./L

LOEC(reproduction): 0.277 mg a.i./L

NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.

Description of key information

One reliable long term study for Isobornyl acrylate on aquatic invertebrates is available acc. OECD 211. NOEC (21d) on Daphnia magna was determined to be 0.092 mg/l.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

NOEC

Effect concentration:

0.092 mg/L