Registration Dossier
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EC number: 202-597-5 | CAS number: 97-63-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- Method and results sufficiently described, with significant technical problems
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to
- Guideline:
- OECD Guideline 475 (Mammalian Bone Marrow Chromosome Aberration Test)
- GLP compliance:
- no
- Type of assay:
- chromosome aberration assay
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- Supplied by ICI; contained 11 ppm hydroquinone stabilizer.
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Animal Breeding Unit, Alderley Park, Cheshire
- Age at study initiation: 8-10 weeks
- Weight at study initiation: 150-200 g
- Housing: four/cage
- Diet (e.g. ad libitum): Alderley Park rat cubes
- Water (e.g. ad libitum): unspecified
ENVIRONMENTAL CONDITIONS
- Air changes (per hr): minimum 8
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- inhalation
- Vehicle:
- unchanged
- Details on exposure:
- whole body
- Duration of treatment / exposure:
- single treatment: 2 hours
repeated treatment: 5 hours - Frequency of treatment:
- repeated treatment: daily for 5 days
- Post exposure period:
- 24 h after the last exposure
Doses / concentrations
- Remarks:
- Doses / Concentrations:
ca. 0.4, 1.6, 2.8 and 4 mg/L (corresponding to 100, 400, 700, 1000 ppm)
Basis:
nominal conc.
- No. of animals per sex per dose:
- 12 animals in the negative control group; 8 animals in each of the treated and positive control groups.
- Control animals:
- yes, concurrent no treatment
- Positive control(s):
- Benzene, 7500 ppm
Examinations
- Tissues and cell types examined:
- Bone marrow
- Details of tissue and slide preparation:
- Bone marrow was collected 24 hours after the single two hour exposure and processed for scoring (blind). Categories used during evaluation included Chromatid or chromsome gaps, Chromatid breaks, Fragments, Minutes or Any other complex abnormality.
- Statistics:
- Proportion of cells with any abnormalities or proportion of cells with any abnormalities other than gaps: Analysis of Variance after transformation of the original data using a double arcsine transformation.
Proportion of rats which had any cells with any abnormality other than gaps: One-sided Fischers Exact test.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- ambiguous
- Toxicity:
- no effects
- Vehicle controls validity:
- not examined
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
The maximum concentration tested caused significant reductions in mitotic activity in the bone marrow of the
exposed animals. Small and non-dose related increases in
the percentages of cells with chromosomal aberrations were
observed in the animals exposed to MMA. However, these
increases were almost exclusively due to gap-type
aberrations and when these were excluded from the data
small increases were only observed at 400 ppm. Such small
increases, observed at lower, but not at higher
concentrations tested, are not considered to be
biologically significant.
For further details, see attached document including 11 tables.
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