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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
Aquatic acute toxicity testing using the nematode Caenorhabditis elegans
Author:
Ura K, Kai T, Sakata S, Iguchi T, Arizono K
Year:
2002
Bibliographic source:
J Health Sci 48: 583-586 (2002)

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
An acute toxicity test was performed according to a procedure of Donkin and Williams (1995). Ten worms (1-day-old larvae) each were dispensed into 24-well tissue culture plates containing 0.5 mL of K-medium and for example benzophenone. All treatments were done in triplicate. No feed was given during the test. The worms were exposed for 24 hr at 20°C and the number of dead worms was determined.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
- Controls: performed
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): DMSO
- Concentration of vehicle in test medium (stock solution and final test solution): <=1%
- Evaporation of vehicle before use: no

Test organisms

Test organisms (species):
Caenorhabditis elegans
Animal group:
nematods
Details on test organisms:
TEST ORGANISM
- Common name: nematode (Caenorhabditis elegans)
- Source: wild type N2 strain
- Age at test initiation (mean and range, SD): 1d
- Weight at test initiation (mean and range, SD): not reported

ACCLIMATION: not reported

Study design

Study type:
laboratory study
Substrate type:
other: K-medium
Limit test:
no
Total exposure duration:
24 h

Test conditions

Test temperature:
20°C
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
56.8 other: mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% c.i.: 52.1-62.1 mg/L
Reported statistics and error estimates:
The median lethal concentration (LC50) was calculated using the PROBIT method.

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Conclusions:
In a static 24 h toxicity test, a LC50=56.8 mg/L (nominal) was determined for the nematode Caenorhabditis elegans.
Executive summary:

An acute toxicity test was performed according to a procedure of Donkin and Williams (1995). Ten worms (1-day-old larvae) each were dispensed into 24-well tissue culture plates containing 0.5 mL of K-medium and for example benzophenone. All treatments were done in triplicate. No feed was given during the test. The worms were exposed for 24 hr at 20°C and the number of dead worms was determined.

In this static 24 h toxicity test, a LC50=56.8 mg/L (nominal) was determined for the nematode Caenorhabditis elegans.