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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Currently viewing:

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
Aquatic acute toxicity testing using the nematode Caenorhabditis elegans
Author:
Ura K, Kai T, Sakata S, Iguchi T, Arizono K
Year:
2002
Bibliographic source:
J Health Sci 48: 583-586 (2002)

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
An acute toxicity test was performed according to a procedure of Donkin and Williams (1995). Ten worms (1-day-old larvae) each were dispensed into 24-well tissue culture plates containing 0.5 mL of K-medium and for example benzophenone. All treatments were done in triplicate. No feed was given during the test. The worms were exposed for 24 hr at 20°C and the number of dead worms was determined.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Benzophenone
EC Number:
204-337-6
EC Name:
Benzophenone
Cas Number:
119-61-9
Molecular formula:
C13H10O
IUPAC Name:
diphenylmethanone

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
- Controls: performed
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): DMSO
- Concentration of vehicle in test medium (stock solution and final test solution): <=1%
- Evaporation of vehicle before use: no

Test organisms

Test organisms (species):
Caenorhabditis elegans
Animal group:
nematods
Details on test organisms:
TEST ORGANISM
- Common name: nematode (Caenorhabditis elegans)
- Source: wild type N2 strain
- Age at test initiation (mean and range, SD): 1d
- Weight at test initiation (mean and range, SD): not reported

ACCLIMATION: not reported

Study design

Study type:
laboratory study
Substrate type:
other: K-medium
Limit test:
no
Total exposure duration:
24 h

Test conditions

Test temperature:
20°C
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
56.8 other: mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% c.i.: 52.1-62.1 mg/L
Reported statistics and error estimates:
The median lethal concentration (LC50) was calculated using the PROBIT method.

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Conclusions:
In a static 24 h toxicity test, a LC50=56.8 mg/L (nominal) was determined for the nematode Caenorhabditis elegans.
Executive summary:

An acute toxicity test was performed according to a procedure of Donkin and Williams (1995). Ten worms (1-day-old larvae) each were dispensed into 24-well tissue culture plates containing 0.5 mL of K-medium and for example benzophenone. All treatments were done in triplicate. No feed was given during the test. The worms were exposed for 24 hr at 20°C and the number of dead worms was determined.

In this static 24 h toxicity test, a LC50=56.8 mg/L (nominal) was determined for the nematode Caenorhabditis elegans.