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EC number: 614-144-2 | CAS number: 67800-94-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline Study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Oligomerisation products of o-Diaminotoluene with ethylene oxide and propylene oxide
- EC Number:
- 614-144-2
- Cas Number:
- 67800-94-6
- Molecular formula:
- C7 H10 N2. ( C3 H6 O )x.(C2 H4 O)y ; 1 < x + y < 8.5
- IUPAC Name:
- Oligomerisation products of o-Diaminotoluene with ethylene oxide and propylene oxide
Constituent 1
Method
- Target gene:
- mutant histidine gene
Species / strainopen allclose all
- Species / strain / cell type:
- other: S. typhimurium TA 1535, TA 100, TA 1537, TA 98
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix was made from the livers of male Sprague Dawley rats, which received a single intraperitoneal injection of 500 mg/kg bw Aroclor 1254, dissolved in corn oil, 5 days prior to sacrifice.
- Test concentrations with justification for top dose:
- standard plate test (SPT):
0, 20, 100, 500, 2500, 5000 µg/plate with and without S9 mix
preincubation assay:
0, 20, 100, 500, 2500, 5000 µg/tube with and without S9 mix - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: deionized water (test item); DMSO (other positive controls)
- Justification for choice of solvent/vehicle: Due to the good solubility of the test substance in water
Controls
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Remarks:
- 4-nitro-1,2-phenylene diamine (TA 98), N-Methyl-N´-nitro-N-nitrososguanidine (only TA 1535, TA 100), 9-aminoacridine (TA 1535), 2-aminoanthracene (TA 1535, TA 100, TA 1537, TA 98, E. coli with S9 mix), 4-nitroquinoline-N-oxide (E. coli)
- Evaluation criteria:
- The lest chemical is considered positive in this assay if the following criteria are met:
• A dose-related and reproducible increase in the number of revertant colonies. i.e.about doubling of the spontaneous mutation rate in at least one tester strain either without 5·9 mix or after adding a metabolizing system.
A test substance is generally considered nonmutagenic in thls test if:
• The number of revertants for all tester strains were within the historical negative control range under all experimental conditions int!wo experiments carried out independently of each other. - Statistics:
- no statistics perfomed; evaluation based on criteria mentioned above
Results and discussion
Test results
- Species / strain:
- other: S. typhimurium TA 1535, TA 100, TA 1537, TA 98, and E. Coli WP2 uvrA
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- A very slight decrease in the number of revertants was occassionally observed depending on the strain and test conditions at doses >= 2500 µg/plate.
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- - Precipitation: No precipitation was found
An increase in number of his+ or trp+ revertants was not observed in the standard plate test or in the preincubation test either without S-9 mix or after the addition of S-9 mix. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Executive summary:
The test substancewas evaluated in an Ames Test on Salmonella typhimurium strains TA 1535, TA 100, TA, 1537, TA 98, and E. coli WP2 uvrA, performed according to OECD TG 471. The test material was considered to be non-mutagenic without and with S9 mix in the standard plate test as well as in the preincubation test.
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