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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Minor restrictions in design and/or reporting but otherwise adequate for assessment.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances):

- Chemical name of vehicle (organic solvent, emulsifier or dispersant): DMSO
Test organisms (species):
Poecilia reticulata
Details on test organisms:
TEST ORGANISM
- Common name: guppy
- Source: Akzo Research Laboratories Arnhem, dpt. CRL.
- Age at study initiation: Young fish
- Length at study initiation: ≤ 2 cm
- The fish were kept at 23 ± 2 ºC
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
None
Hardness:
13°dH
Test temperature:
23 ± 2°C
pH:
approximately 8.2
Salinity:
Not applicable
Nominal and measured concentrations:
Not known
Details on test conditions:
TEST SYSTEM
- Test vessel: 2L glass aquaria
- Material, size, headspace, fill volume: 2 l glass aquaria filled with 1 l of test medium.
- Aeration: The medium was aerated before use.
- No. of organisms per vessel: 7-10 fish per concentration
- Renewal rate of test solution : The test medium was refreshed after 48 hours.
- Biomass loading rate: The loading in the glass test vessels was approximately 0.6 g biomass/l.

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic medium, Dutch Standard Water according to NPR 6507
- Containing: 100 mg NaHCO3/l, 20 mg KHCO3/l, 200 mg CaCl2.2H2O/l and 180 mg MgSO4.7H2O/l
- Preparation of the test medium: A stock solution in DMSO was made, from which a second stock solution in dilution water was prepared.
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Mortality of control: ≤ 10%

Description of key information

The 96-h LC50 of >10 mg/L in Poecilia reticulata will be used in the assessment.

Key value for chemical safety assessment

Additional information

One short-term toxicity study with freshwater fish is available for zinc bis(dibenzyldithiocarbamate). The test is a semi-static non-GLP, OECD 203 guideline study with Poecilia reticulata without analytical monitoring (Akzo Nobel Research Laboratories, 1991). The 96-h LC50 value is >10 mg/L.