Disodium wolframate

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Insufficient information provided on methods to accurately evaluate the study. The study does not mention OECD guidelines or GLP compliance. The details of the environmental conditions for the animals were not discussed.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1965

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The acute toxicity of sodium tungstate was evaluated following intraperitoneal injection

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Disodium wolframate

EC Number:

236-743-4

EC Name:

Disodium wolframate

Cas Number:

13472-45-2

Molecular formula:

Na2WO4

IUPAC Name:

disodium dioxotungstenbis(olate)

Details on test material:

- Name of test material (as cited in study report): Sodium tungstate

Test animals

Species:

mouse

Strain:

Swiss

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Mus musculus, albinos variety
- Weight at study initiation: 20+/- 2 g

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

physiological saline

Details on exposure:

The oxygenated salt had been placed in solution in physiological saline in a concentration such that the volume of liquid injected was not too high, so as to avoid shock. The animals were administered intraperitoneally in a "single dose".

Doses:

No data

No. of animals per sex per dose:

No data

Control animals:

yes

Details on study design:

The test substance was placed in solution in physiological saline and administered to the animals intraperitoneally in a single dose. The maximum-dose-never-fatal (MDNF) in 24 hours was determined, that is, the largest dose not causing any mortality. The minimum-dose-always-fatal (mDAF) in 24 hours was determined, that is, the smallest dose provoking 100% mortality in 24 hours. The mortality percentage using 10 intermediary doses between the maximum-dose-never fatal and the minimum- doses- always- fatal was then evaluated. The LD50's were calculated using the method of Kaerber and Behrens and Litchfield and Wilcoxon.
The animals were then isolated and placed under observation in strictly identical conditions (food, temperature, etc.) for 90 days. The mortality percentage was recorded each day, the behavior and state of health of the animals were observed and the LD50's were calculated. Simultaneously with the actual toxicity studies, blanks were performed in which animals of the same age and of the same weight as those dosed with the test substance received the same volume of physiological serum as that administered to the test animals by the intraperitoneal route.

Statistics:

The LD50's were calculated using the method of Kaerber and Behrens and Litchfield and Wilcoxon.

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

0.47 other: mM/kg

Remarks on result:

other: calculated using the method of Kaerber and Behrens

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

0.44 other: mM/kg

Remarks on result:

other: calculated using the method of Litchfield and Wilcoxon

Mortality:

See below for mortality and dismal mortality results.

Clinical signs:

Mice showed symptomatology during sodium tungstate intoxication. Sodium tungstate provoked prostration and a state of lethargy.

Body weight:

no data

Gross pathology:

no data

Other findings:

Sodium tungstate did not seem to show delayed toxicity. Nevertheless, Sodium tungstate's lethal action is fairly slow and 48 hours are necessary for it to manifest lethal action. After 48 hours, the animals recover completely and their growth is normal. They do not show any apparent traces of the poisoning to which they have been exposed.

Any other information on results incl. tables

- The LD50 for mice is 135.27 mg/kg for Sodium tungstate dihydrate.

Mortality after 24 hours                                                                                    

MDNF: 0.30 mM/kg

LD50 (K&B): 0.47 mM/kg

LD50 (L&W): 0.44 mM/kg

mDAF: 0.60 mM/kg

Dismal Mortality

Infralethal dose: 0.30 mM/kg

Limit LD50 (K&B): 0.44 mM/kg

Limit LD50 (L&W): 0.41 mM/kg

Per. of crisis in days: 2

Applicant's summary and conclusion

Conclusions:

Sodium tungstate provoked a very prompt response in mice. The period of crisis was 2 days. The i.p. LD50 for male mice was determined to be 0.44- 0.47 mM/kg-bw (dependent on method of calculation).