2-imidazolidone

Toxicity to other aquatic organisms

Administrative data

Endpoint:

toxicity to other aquatic vertebrates

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Remarks:

The puritiy of the test substance is not provided in the publication. Additionally, the test concentrations used were by far higher than the threshold concentration at 100 mg/L for the acute aquatic test design.

Data source

Reference

Reference Type:

publication

Title:

Actions of antifertility chemicals on Xenopus laevis spermatozoa in vitro

Author:

Jones P and Jackson H

Year:

1973

Bibliographic source:

J. Reprod. Fert. (1974) 38, 347-357

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

A sperm suspension (0.3 mL) was thoroughly mixed with a solution (1.2 mL) of the test substance over a range of concentrations; a control was also included.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-imidazolidone

EC Number:

204-436-4

EC Name:

2-imidazolidone

Cas Number:

120-93-4

Molecular formula:

C3H6N2O

IUPAC Name:

2-imidazolidone

Test material form:

solid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Xenopus laevis

Details on test organisms:

HOUSING
- Vessels: Polypropylene boxes measuring 17.5 x 11 x 5 inches filles with 1.25 to 1.5 gallons of tap water
- Number of animals per vessel: 3 - 4
- Temperature: 22 - 24 °C
- Feeding: Twice a week with chopped liver

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

120 min

Test conditions

Test temperature:

22 °C

Nominal and measured concentrations:

Nominal: 125, 250, 500, 1000 and 2000 mg/L

Results and discussion

Any other information on results incl. tables

Very high concentrations of ethyleneurea on Xenopus spermatozoa produced abnormal embryos. Over the concentration range of 2000 to 125 mg/L, cleavage rates were high at all levels after 30 and 60 minutes of exposure. The majority of eggs developed abnormally at the highest concentration (2000 mg/L). At lower dose levels, the toxic effect rapidly disappeared and after exposure for 60 and 120 min normal development was clearly dose-dependent. No effects were observed at a concentration of 125 mg/L.