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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally accepted scientific standards with acceptable restirctions.
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
publication
Title:
Acute (Rate and Mouse) and Short-term (Rat) Toxicity Studies on cis-3-Hexen-1-ol
Author:
Gaunt IF, Colley J, Grasso P, Lansdown ABG, Gangolli SD
Year:
1969
Bibliographic source:
Fd Cosmet. Toxicol. 7: 451-459
Report Date:
1969

Materials and methods

Principles of method if other than guideline:
Undiluted cis-3-hexen-1-ol was given by intraperitoneal injection to groups of 5 mice of each sex.
GLP compliance:
no
Remarks:
Study predates GLP.
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Details on test material:
- Name of test material (as cited in study report): cis-3-hexen-1-ol
- Substance type: flavouring in foodstuffs, particularly in baked goods
- Physical state: no data
- Analytical purity: 96 %
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: 96 % pure, the balance bring the tans-isomer
- Isomers composition: 96 % pure, the balance bring the tans-isomer
- Purity test date: no data
- Lot/batch No.: no data
- Expiration date of the lot/batch: no data
- Stability under test conditions: no data
- Storage condition of test material: no data

Test animals

Species:
mouse
Strain:
other: CFW
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Age at study initiation: weanling
- Weight at study initiation: 150- 200 g
- Fasting period before study: overnight
- Housing: 5 to a cage
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
unchanged (no vehicle)
Doses:
No data.
No. of animals per sex per dose:
5/sex/dose
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 7 days
- Necropsy of survivors performed: yes
Statistics:
The LD50 values were calculated by the method of Litchfield & Wilcoxon (1949).

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
>= 0.4 - <= 0.6 other: g/kg
Based on:
test mat.
Remarks on result:
other: No consistent sex differences.
Mortality:
Most deaths occured within 3 hr of treatment and all within 24 hr. At higher doses the animlas died within a few minutes.
Clinical signs:
Ataxia followed by cessation of spontaneous movement, coma and death. At higher doses the animlas became comatose within seconds of treatement.
Body weight:
No data.
Gross pathology:
No abnormalities were noted at autopsy.

Any other information on results incl. tables

The LD50 values show that there is no significant species or sex difference in the acute toxicity of the test substance. The wide difference in acute toxicity between oral and intraperitoneal dosage could be due to poor or slow absorption from the gastro-intestinal tract, to rapid metabolism in the intestine or liver before the test substance reaches the general circulation or to peritonitis following intraperitoneal injection. 

Applicant's summary and conclusion

Conclusions:
The test substance was assessed for acute toxicity via intraperitoneal injection and was found to have an LD50 of 0.4- 0.6 g/kg.