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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 70 mg, 80 mg and 90mg of the test substance in 10 liters deionized water with continuous stirring for achieving test concentrations of 7 mg/L, 8 mg/L and 9mg/L, respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Ashish Fish Center
- Length at study initiation (length definition, mean, range and SD): average 2.3 cm
- Weight at study initiation (mean and range, SD): average 0.203 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12 hours darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Test temperature:
30 °C
pH:
7
Conductivity:
0.5 to 1 µS/cm
Nominal and measured concentrations:
7 , 8 , 9 , 11 , 12.5 , 25 , 50, 100 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12 hours darkness

Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
11 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

8.1. Mortality and symptoms

Nominal conc. (mg/l)

Number of fish

Mortality (cumulated) after

24h

48h

72h

96h

0 (control)

8

0

0

0

0

7

8

0

0

0

0

8

8

0

0

0

0

9

8

0

4

0

0

11

8

8

         8

-

-

12.5

8

8

-

-

-

25

8

8

-

-

-

50

8

8

-

-

-

100

8

8

-

-

-

Nominal conc. (mg/l)

Number of fish

Symptoms after

24h

48h

72h

96h

0 (control)

8

NS8

NS8

NS8

NS8

7

8

NS8

NS8

NS8

NS8

8

8

NS8

NS8

NS8

NS8

9

8

M8

M4

NS4

NS4

11

8

-

-

-

-

12.5

8

-

-

-

-

25

8

-

-

-

-

50

8

-

-

-

-

100

8

-

-

-

-

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

 

8.2. pH and oxygen content:

Nominal conc. (mg/l)

pH readings after

24h

48h

72h

96h

0 (control)

6.3

6.3

6.2

6.0

7

6.0

6.0

5.3

5.0

8

6.0

6.0

5.0

4.6

9

6.0

6.0

5.9

5.8

11

6.0

-

-

-

Nominal conc. (mg/l)

Oxygen content (mg/l) after

24h

48h

72h

96h

0 (control)

7.5

7.5

7.3

7.0

7

7.3

7.3

6.9

6.5

8

7.2

7.2

6.7

6.5

9

7.5

7.3

7.3

6.5

11

7.4

-

-

-

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test material on Zebra Fish Danio rerio was determine to be 9 mg/l.
Executive summary:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 70 mg, 80 mg and 90mg of the test substance in 10 liters deionized water with continuous stirring for achieving test concentrations of 7 mg/L, 8 mg/L and 9mg/L, respectively and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.The nominal concentarion were used 7 , 8 , 9 , 11 , 12.5 , 25 , 50, 100 mg/l .After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be 9 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 2 as per the CLP classification criteria.

Description of key information

Short-term toxicity to fish:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 70 mg, 80 mg and 90mg of the test substance in 10 liters deionized water with continuous stirring for achieving test concentrations of 7 mg/L, 8 mg/L and 9mg/L, respectivelyand Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.The nominal concentarion were used7 , 8 , 9 , 11 , 12.5 , 25 , 50, 100 mg/l .After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be 9 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 2 as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
9 mg/L

Additional information

Short-term toxicity to fish:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 70 mg, 80 mg and 90mg of the test substance in 10 liters deionized water with continuous stirring for achieving test concentrations of 7 mg/L, 8 mg/L and 9mg/L, respectivelyand Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.The nominal concentarion were used7 , 8 , 9 , 11 , 12.5 , 25 , 50, 100 mg/l .After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be 9 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 2 as per the CLP classification criteria.