Cinnamyl alcohol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 70 mg, 80 mg and 90mg of the test substance in 10 liters deionized water with continuous stirring for achieving test concentrations of 7 mg/L, 8 mg/L and 9mg/L, respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Ashish Fish Center
- Length at study initiation (length definition, mean, range and SD): average 2.3 cm
- Weight at study initiation (mean and range, SD): average 0.203 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12 hours darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

30 °C

pH:

7

Conductivity:

0.5 to 1 µS/cm

Nominal and measured concentrations:

7 , 8 , 9 , 11 , 12.5 , 25 , 50, 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12 hours darkness

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

11 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

8.1. Mortality and symptoms

Nominal conc. (mg/l)	Number of fish	Mortality (cumulated) after
		24h	48h	72h	96h
0 (control)	8	0	0	0	0
7	8	0	0	0	0
8	8	0	0	0	0
9	8	0	4	0	0
11	8	8	8	-	-
12.5	8	8	-	-	-
25	8	8	-	-	-
50	8	8	-	-	-
100	8	8	-	-	-

Nominal conc. (mg/l)	Number of fish	Symptoms after
		24h	48h	72h	96h
0 (control)	8	NS8	NS8	NS8	NS8
7	8	NS8	NS8	NS8	NS8
8	8	NS8	NS8	NS8	NS8
9	8	M8	M4	NS4	NS4
11	8	-	-	-	-
12.5	8	-	-	-	-
25	8	-	-	-	-
50	8	-	-	-	-
100	8	-	-	-	-

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

 

8.2. pH and oxygen content:

Nominal conc. (mg/l)	pH readings after
	24h	48h	72h	96h
0 (control)	6.3	6.3	6.2	6.0
7	6.0	6.0	5.3	5.0
8	6.0	6.0	5.0	4.6
9	6.0	6.0	5.9	5.8
11	6.0	-	-	-

Nominal conc. (mg/l)	Oxygen content (mg/l) after
	24h	48h	72h	96h
0 (control)	7.5	7.5	7.3	7.0
7	7.3	7.3	6.9	6.5
8	7.2	7.2	6.7	6.5
9	7.5	7.3	7.3	6.5
11	7.4	-	-	-

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test material on Zebra Fish Danio rerio was determine to be 9 mg/l.

Executive summary:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 70 mg, 80 mg and 90mg of the test substance in 10 liters deionized water with continuous stirring for achieving test concentrations of 7 mg/L, 8 mg/L and 9mg/L, respectively and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.The nominal concentarion were used 7 , 8 , 9 , 11 , 12.5 , 25 , 50, 100 mg/l .After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be 9 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 2 as per the CLP classification criteria.

Description of key information

Short-term toxicity to fish:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 70 mg, 80 mg and 90mg of the test substance in 10 liters deionized water with continuous stirring for achieving test concentrations of 7 mg/L, 8 mg/L and 9mg/L, respectivelyand Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.The nominal concentarion were used7 , 8 , 9 , 11 , 12.5 , 25 , 50, 100 mg/l .After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be 9 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 2 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

9 mg/L

Additional information

Short-term toxicity to fish:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 70 mg, 80 mg and 90mg of the test substance in 10 liters deionized water with continuous stirring for achieving test concentrations of 7 mg/L, 8 mg/L and 9mg/L, respectivelyand Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203.The nominal concentarion were used7 , 8 , 9 , 11 , 12.5 , 25 , 50, 100 mg/l .After 96 hours of exposure to test item to various nominal test concentrations, LC50 was determine to be 9 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified as aquatic chronic 2 as per the CLP classification criteria.