Tris(p-isocyanatophenyl) thiophosphate

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Tris(p-isocyanatophenyl) thiophosphate

EC Number:

223-981-9

EC Name:

Tris(p-isocyanatophenyl) thiophosphate

Cas Number:

4151-51-3

Molecular formula:

C21H12N3O6PS

IUPAC Name:

O,O,O-tris-(4-isocyanatophenyl) thiophosphate

Test animals / tissue source

Species:

rabbit

Strain:

Himalayan

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Sex: female
- Source: LPT Laboratory of Pharmacology and Toxicology KG, Löhndorf, Germany
- Age at study initiation: appr. 3.5 months
- sex: males

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: one untreated eye of test animals served as control

Amount / concentration applied:

Amount: 0.1 ml

Duration of treatment / exposure:

single treatment; the lid was held together for about one second after instillation

Observation period (in vivo):

the eyes were examined ophthalmoscopically with a slit lamp prior to the administration and also 1, 24, 48, 72 hours post application. 24 hours after administration the eyes were treated additionally with fluorescein and examined.

Number of animals or in vitro replicates:

3

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing: none

SCORING SYSTEM: Draize

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Remarks:

cornea, iris, and conjunctivae

Basis:

mean

Time point:

other: 24 h, 48 h, 72 h

Score:

0

Max. score:

4

Remarks on result:

other: 1 hour after instillation redness of conjunctivae (grade 1; all animals), chemosis (grade 1; 1 animal) , and cornea opacity (grade 1; 2 animals) was observed

Other effects:

There were no systemic intolerance reactions.

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information

Conclusions:

Classification: not irritating

Executive summary:

In a study performed according to OECD 405 the test item (27% tris(p-isocyanatophenyl)thiophosphate in ethyl acetate)

did not induce irritant effects in the eye of rabbits. There were no systemic intolerance reactions.