Diethoxymethane

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Lab not following OECD guidelines or GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Internal SOP: TA300, TA120

Deviations:

not specified

Principles of method if other than guideline:

dose level tested: 5000, 2500 and 1250 mg/kg. Administrated as received. method of calculation: Weil method

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Diethoxymethane

EC Number:

207-330-6

EC Name:

Diethoxymethane

Cas Number:

462-95-3

Molecular formula:

C5H12O2

IUPAC Name:

diethoxymethane

Test animals

Species:

rat

Strain:

other: CRL: CD (SD) BR

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Doses:

5000, 2500 and 1250 mg/kg

No. of animals per sex per dose:

5

Control animals:

not specified

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

3 536 mg/kg bw

Based on:

test mat.

95% CL:

2 400 - 5 209

Mortality:

4/5 individuals (male and females) at 5000 mg/kg.
None at the other dose levels

Clinical signs:

other: Ataxia: at all doses, immediate to 1 hour Weaknes slight to severe: at all doses, immediate to 4 hours Prostration: at 5000 mg/kg, 1hour to 4 hours Normal state: at all doses, day 1 to day 14

Gross pathology:

none

Any other information on results incl. tables

CLINICAL SIGNS
SIGN	DOSE(mg/kg)	TIME	SEX
Ataxia	5000, 2500, 1250	Immediate to1 Hour	M,F
Weakness-Slightto Severe	5000, 2500, 1250	Immediate to 4 Hours	M,F
Prostration	5000	1 Hour to 4 Hours	M,F
Normal	5000, 2500, 1250	Day 1 to Day14	M,F

The test material was, at most, slightly toxic by the oral route. Abnormal clinical signs included slight to severe weakness at all dose levels, ataxia at the first hour after dosing, and prostration up to four hours after dosing at the high dose level. Eight of ten animals administered the high (5000 mg/kg) dose died within 24 hours, but all survivors appeared clinically normal from Day 1 through the observation period. The cause of death in animals dying after exposure to the test material was not determined. Treatment-related changes in those animals dying within one day of dosing included congestion, edema, and hemorrhage of the glandular gastric mucosa and reddish blue urine in the urinary bladder. No abnormalities related to administration of the test material were noted in animals surviving the observation period.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The Gavage LD50 of Ethylal on rats is 3536 mg/kg which is superior to the CLP cut off values of 2000 mg/kg

Executive summary:

The Gavage LD50 of Ethylal on rats is 3536 mg/kg which is superior to the CLP cut off values of 2000 mg/kg