trisodium 2-(2-sulfoethoxy)ethane-1-sulfonate ethenesulfonate

Hydrolysis

Administrative data

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented study according OECD 111 and GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 111 (Hydrolysis as a Function of pH)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 835.2110 (Hydrolysis as a Function of pH)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

trisodium 2-(2-sulfonatoethoxy)ethane-1-sulfonate ethenesulfonate

EC Number:

700-978-5

Molecular formula:

C2H3O3S.Na C4H8O7S2Na2

IUPAC Name:

trisodium 2-(2-sulfonatoethoxy)ethane-1-sulfonate ethenesulfonate

Details on test material:

- Name of test material (as cited in study report): Sodium vinyl sulphonate solution (Provichem 2202)
- Analytical purity: 25.09%
- Lot/batch No.: 200911040014
- Storage condition of test material: Ambient room temperature 20-30°C

Study design

Analytical monitoring:

yes

Details on sampling:

- Sampling intervals for the parent/transformation products: hydrolysis samples were collected from each pH after the test solution was placed into the test vessels (0h) and at 2h, 5h, 1d, 5d.
- Sampling method:At each sampling interval for each buffer test system, duplicate samples were taken and analyzed by HPLC.
- Sampling intervals/times for pH measurements:The pH of the buffer solutions were measured after preparation and sterile filtration. The pH was also documented after sample fortification of pH 4, 7 and 9 buffer test solution on Day 0 and Day 5.
- Sampling intervals/times for sterility check: Sterility was determined at Day 0 for sterile pH 4, 7 and 9 buffer test systems and at day 5 for pH 4, 7 and 9 buffer test system. Sterility was checked by serial dilution plate technique. Agar plates (Nutrient agar for bacteria) were used to determine sterility of buffer test systems.
-Samples were analyzed on the sampling day

Buffers:

- pH: 4, 7, 9
- Type and final molarity of buffer,Composition of buffer:
pH4: 0.01 M: 410 mL of 0.01 M acetic acid solution with 90 mL of 0.01 M sodium acetate solution
pH7: 0.02M: 195 mL of 0.02M sodium phosphate (monobasic) and 305 mL of 0.02M sodium phosphate (dibasic) to a 1000mL flask
pH9: 0.5M boric acid:

Details on test conditions:

TEST MEDIUM
- Volume used/treatment: nominal test item concentration was 50 mg/L
- Kind and purity of water: Milli-Q-water
- Preparation of test medium: dilution of stock solution with filter-sterilized buffer solution to obtain 50 mg/L

Duration of test

Duration of test 1

Duration:

0 h

pH:

4

Temp.:

50 °C

Initial conc. measured:

49.9 mg/L

Duration of test 2

Duration:

0 h

pH:

7

Temp.:

50 °C

Initial conc. measured:

50.3 mg/L

Duration of test 3

Duration:

0 h

pH:

9

Temp.:

50 °C

Initial conc. measured:

50.6 mg/L

Number of replicates:

2 replicates per test system

Positive controls:

not specified

Negative controls:

not specified

Statistical methods:

The base 10 logarithm of the concentration of SVS was plotted as a function of time and the line fit to the data was determined using Excel.

Results and discussion

Preliminary study:

Analysis of pH4 buffer at Day 5 showed >90% of the recovered test item compared to the 0h analysis. Analysis of pH7 buffer at Day 5 showed >90% of the recovered test item compared to the 0h analysis. Analysis of pH9 buffer at Day 5 showed >90% of the recovered test item compared to the 0h analysis. SVS was hydrolytically stable at pH 4, 7, and 9 based on preliminary result. So definitive study was not conducted.

Transformation products:

no

Remarks:

Sodium vinyl sulphonate was hydrolytical stable at pH 4, pH 7 and pH 9 based on the preliminary result.A definitive study was not conducted

Total recovery of test substance (in %)

Total recovery of test substance (in %) 1

% Recovery:

> 90

pH:

4

Temp.:

50 °C

Duration:

5 d

Total recovery of test substance (in %) 2

% Recovery:

> 90

pH:

7

Temp.:

50 °C

Duration:

5 d

Total recovery of test substance (in %) 3

% Recovery:

> 90

pH:

9

Temp.:

50 °C

Duration:

5 d

Dissipation DT50 of parent compound

Dissipation DT50 of parent compound 1

Key result

pH:

9

Temp.:

50 °C

Remarks on result:

hydrolytically stable based on preliminary test

Remarks:

Analysis at pH 9 of the parent component after 5days showed >90 % of the recovered test item compared to the 0 hour analysis.

Dissipation DT50 of parent compound 2

Key result

pH:

7

Temp.:

50

Remarks on result:

hydrolytically stable based on preliminary test

Remarks:

Analysis at pH 7 of the parent compound after 5 days showed >90 of the recovered test item compared to the 0 hour analysis

Dissipation DT50 of parent compound 3

Key result

pH:

4

Temp.:

50 °C

Remarks on result:

hydrolytically stable based on preliminary test

Remarks:

Analysis of the parent compound at day 5showed >90 % of the recovered test item compared to the 0 hour analysis

Applicant's summary and conclusion

Conclusions:

This study demonstrated that Sodium vinyl sulphonate solution (provichem 2202) was hydrolitically stable at pH 4, 7 and 9. Based on the results of this study, hydrolysis will not be a route of degradation of SVS in the environment.