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EC number: 801-656-8 | CAS number: 1416808-92-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- (3E)-2-chloro-3-(hydroxymethylidene)cyclohex-1-ene-1-carbaldehyde
- EC Number:
- 801-656-8
- Cas Number:
- 1416808-92-8
- Molecular formula:
- C8H9ClO2
- IUPAC Name:
- (3E)-2-chloro-3-(hydroxymethylidene)cyclohex-1-ene-1-carbaldehyde
- Test material form:
- other: yellow solid molten
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- Dose range finding: up to 5000 µg/plate in the absence and presence of S9-mix in the strains TA100 and WP2uvrA
Main test: up to limited concentrations/plate because of observed toxicity in dose range finding.
Controls
- Untreated negative controls:
- yes
- Remarks:
- DMSO
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- 2-nitrofluorene
- sodium azide
- methylmethanesulfonate
- other: ICR-191 (Acros Organics, Geel, Belgium); 2-aminoanthracene (only control with all strains in presence of S9 mix)
Results and discussion
Test resultsopen allclose all
- Species / strain:
- bacteria, other: DOSE RANGE FINDING TEST: Salmonella typhimurium TA100 and Escherichia coli WP2uvrA
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- TA100: in the absence and presence of S9-mix
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- TA100: at 512 μg/plate and above in the absence of S9-mix and at 1600 and 5000 μg/plate in the presence of S9-mix; WP2uvrA: at 1600 and 5000 μg/plate in the absence and presence of S9-mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- bacteria, other: FIRST MAIN TEST: TA1535, TA1537 and TA98
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- TA98: in the presence of S9-mix
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- TA1535, TA1537 and TA98
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- bacteria, other: SECOND MAIN TEST: TA100, TA1535, TA1537, TA98 and WP2uvrA
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- TA100: in the absence and presence of S9-mix; TA98: in the presence of S9-mix
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- TA1535, TA1537 and TA98 in the absence of S9-mix and TA100 in the absence and presence of S9-mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- bacteria, other: THIRD MAIN TEST: TA1535, TA1537, TA98 and WP2uvrA
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- TA98: in the presence of S9-mix
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- all tester strains, except WP2uvrA in the presence of S9-mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- bacteria, other: FOURTH MAIN TEST: WP2uvrA
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Remarks:
- in the presence of 10% (v/v) S9-mix and up to 5000 µg/plate
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- in the presence of 10% (v/v) S9-mix and up to 5000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Since 1.7- to 2.9-fold, dose related increases were observed in two tester strains, in the absence (TA100) and presence of S9-mix (TA98 and TA100) and the results were reproducible in the repeat experiments, these increases are biologically relevant and the substance is considered to be mutagenic in the absence and presence of S9-mix.
All other bacterial strains showed negative responses over the entire dose range, i.e. no dose-related, increase in the number of revertants in follow-up experiments.
In this study, the negative and strain-specific positive control values were within the laboratory historical control data ranges indicating that the test conditions were adequate and that the metabolic activation system functioned properly.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results : positive
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