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EC number: 500-002-6 | CAS number: 9002-92-0 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- data from handbook or collection of data
Data source
Reference
- Reference Type:
- publication
- Title:
- SALMONELLA MUTAGENICITY TESTS
- Author:
- ZEIGER,E et al.
- Year:
- 1 987
- Bibliographic source:
- ENVIRON. MOL. MUTAGEN.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other:
- Principles of method if other than guideline:
- Preincubation Assay
The preincubation assay was performed . The test chemical, Salmonella culture, and S-9 mix or buffer were incubated at 37"C, without shaking, for 20 min. Chemicals known or suspected to be volatile were incubated in capped tubes. The top agar was added, and the contents of the tubes were mixed and poured onto the surface of petri dishes that contained Vogel-Bonner medium [Vogel and Bonner, 19561. The histidine-revertant (his') colonies arising on these plates were counted following 2 days incubation at 37°C. The plates were hand-counted when a precipitate was present; otherwise automatic colony counters were used.
All chemicals were tested initially in a toxicity assay to determine the appropriate dose range. The toxicity assay was performed by using TA100 or the system.
Toxic concentrations were those at which a decrease in the number of hisf colonies was seen or at which there was a clearing in the density of the background lawn.At least five doses of the chemical were tested in triplicate. Experiments were repeated at least 1 wk following the initial trial. Each chemical was tested initially at half-log doses up to a dose that elicited toxicity; subsequent trials occasionally used narrower dose increments. Chemicals that were not toxic were tested to a maximum dose of 10 mglplate. Chemicals that were poorly soluble were tested up to a dose defined by their solubility. A maximum of 0.05 ml solvent was added to each plate.Concurrent solvent and positive controls were run with each trial. The positive controls in the absence of metabolic activation were sodium azide (TA1535 and TA loo), 9-aminoacridine (TA97 and TA 1S37), and 4-nitro-o-phenylenediamine (TA98). The positive control for metabolic activation was 2-aminoanthracene for all strains. Although there were no specific response ranges established for the solvent and positive controls, each laboratory rejected experiments in which the positive control chemical did not produce a mutagenic response or in which the solvent controlvalues were higher (or lower in the case of TAlOO and TA97) than their expected values.During the initial stages of the testing program, chemicals were tested with 10% S-9. Other levels of S-9 were used when an equivocal result was obtained with 10%. The protocol evolved to one that used 30% S-9 when a negative response was obtained with 10% S-9. - GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dodecan-1-ol, ethoxylated
- EC Number:
- 500-002-6
- EC Name:
- Dodecan-1-ol, ethoxylated
- Cas Number:
- 9002-92-0
- Molecular formula:
- C58H118024
- IUPAC Name:
- Dodecan-1-ol, ethoxylated
- Test material form:
- liquid
- Details on test material:
- Name: Dodecyl alcohol, ethoxylated
CAS No.: 9002-92-0
Molecular Formula: (C2-H4-O)mult-C12-H26-O
Molecular Weight: 230.389 g/mol
Constituent 1
Method
- Target gene:
- histidine-manufacturing gene.
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: All the bacterial strains used in the Ames test carry a defective (mutant) gene that prevents them from synthesizing the essential amino acid histidine from the ingredients in standard bacterial culture medium.
- Metabolic activation:
- with and without
- Metabolic activation system:
- 10% HLI =induced male Syrian hamster liver S9 ; 10% RLI = induced male Sprague Dawley rat liver S9
- Test concentrations with justification for top dose:
- 0,1,3,10,33,100,333 and 1000 µg/Plate
- Vehicle / solvent:
- Vehicle Control : Dimethyl Sulfoxide
Controlsopen allclose all
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- Positive control for TA 100 and TA 1535 tested in the absence of S9 Migrated to IUCLID6: without S9
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Water
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- Positive control for TA 98 tested in the absence of S9
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Water
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Positive control for TA 1537 tested in the absence of S9
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Water
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (or occasionally, sterigmatocystin)
- Remarks:
- For all strains(TA 100,TA 1535, TA1537, TA98) tested with S9
- Details on test system and experimental conditions:
- All chemicals were tested in Salmonella typhimurium strains TA98, TA100,TA1535, and TA1537 and/or TA97. The majority of chemicals were tested in TA1537,and a few were tested in TA97. The testing in both strains is the result of an evolution of the protocol described by Haworth et a1 [1983]. In this original protocol, TA1537 was used. In a later protocol, TA97 replaced TA1537, but the option to retest a chemical in TA1537 was retained for chemicals that produced a positive or questionable response in TA97 and negative responses in the other strains.All strains were obtained from Dr. Bruce Ames and were stored as recommended [Maron and Ames, 19831. Prior to their use for mutagenicity assays, all cultures were grown overnight with shaking at 37°C in Oxoid broth, and their phenotypes were analyzed.
- Evaluation criteria:
- The histidine-revertant (his') colonies were observed for mutagenicity.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- with 10% HLI and 10% RLI
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- with 10% HLI and 10% RLI
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- with 10% HLI and 10% RLI
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- with 10% HLI and 10% RLI
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
TA 100
Dose |
No Activation
(Negative) |
No Activation
(Weakly Positive) |
10% RLI
(Negative) |
10% RLI
(Negative) |
10% HLI
(Negative) |
10% HLI
(Negative) |
||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Protocol | Preincubation | Preincubation | Preincubation | Preincubation | Preincubation | Preincubation | ||||||
ug/Plate | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM |
0 |
99 | 2 | 98 | 3.8 | 137 | 1.7 | 94 | 5.5 | 143 | 5.5 | 111 | 7 |
1 |
119 | 7 | ||||||||||
3 |
95 | 3.2 | 132 | 3.8 | 115 | 6.5 | 122 | 8.7 | ||||
10 |
98 | 6 | 137 | 5 | 164 | 7.4 | 107 | 3.7 | 139 | 9.7 | 123 | 11.8 |
33 |
97 | 8.6 | 120 | 8.8 | 151 | 8.2 | 127 | 1.8 | 135 | 4.6 | 120 | 5.2 |
100 |
61 | 5.3 | 105 | 4.6 | 157 | 3 | 111 | 8.1 | 146 | 7.7 | 116 | 11 |
333 |
0 | 0 | 139 | 8 | 60 | 6.2 | 136 | 19.7 | 85 | 12.5 | ||
1000 |
23 | 4.4 | 11 | 2.2 | ||||||||
Positive Control | 322 | 6 | 405 | 7.1 | 885 | 103.7 | 419 | 26.3 | 1065 | 143.4 | 1204 | 108.6 |
TA 1535
Dose |
No Activation
(Negative) |
No Activation
(Negative) |
10% RLI
(Negative) |
10% RLI
(Negative) |
10% HLI
(Negative) |
10% HLI
(Negative) |
||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Protocol | Preincubation | Preincubation | Preincubation | Preincubation | Preincubation | Preincubation | ||||||
ug/Plate | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM |
0 |
25 | 3.8 | 20 | .9 | 11 | 1.5 | 7 | .9 | 6 | 1.3 | 7 | 2.3 |
1 |
29 | 1.5 | ||||||||||
3 |
29 | 1.5 | 26 | 4.2 | 7 | .6 | 10 | 1.5 | ||||
10 |
29 | 4 | 33 | 2.9 | 11 | 1.5 | 10 | 1.5 | 10 | 1.5 | 5 | 2 |
33 |
23 | 4.3 | 27 | 4.9 | 13 | .7 | 9 | 2.3 | 9 | 3.5 | 11 | 1.7 |
100 |
32 | 3.2 | 19 | 4.8 | 9 | 2.7 | 7 | 1.2 | 13 | .7 | 12 | 1.8 |
333 |
14 | .9 | 7 | .9 | 7 | .7 | 12 | 1.7 | 7 | 2 | ||
1000 |
8 | .9 | 6 | 2 | ||||||||
Positive Control | 452 | 12.8 | 352 | 41.8 | 162 | 11.8 | 183 | 11.5 | 437 | 22.8 | 502 | 41 |
Dose |
No Activation
(Negative) |
No Activation
(Negative) |
10% RLI
(Negative) |
10% RLI
(Negative) |
10% HLI
(Negative) |
10% HLI
(Negative) |
||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Protocol | Preincubation | Preincubation | Preincubation | Preincubation | Preincubation | Preincubation | ||||||
ug/Plate | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM |
0 |
3 | 1.3 | 5 | 1 | 9 | 2.2 | 4 | .7 | 7 | 1.9 | 4 | .9 |
1 |
7 | 2.5 | ||||||||||
3 |
7 | .9 | 3 | .6 | 8 | 2.1 | 10 | 2 | ||||
10 |
7 | 1.3 | 5 | 2.3 | 6 | .9 | 8 | 2.4 | 8 | 2.3 | 6 | .3 |
33 |
6 | 1.9 | 3 | .6 | 8 | .6 | 9 | 1.5 | 7 | 2.8 | 8 | .7 |
100 |
9 | 1.3 | 4 | .7 | 10 | 1.2 | 6 | .9 | 7 | .3 | 8 | 1.2 |
333 |
2 | .9 | 4 | 1.2 | 6 | .6 | 9 | 2.1 | 6 | .3 | ||
1000 |
4 | .3 | 4 | .6 | ||||||||
Positive Control | 149 | 27.8 | 110 | 9.7 | 116 | 23.4 | 122 | 17.1 | 409 | 14.9 | 246 | 27.1 |
Dose |
No Activation
(Negative) |
No Activation
(Negative) |
10% RLI
(Negative) |
10% RLI
(Negative) |
10% HLI
(Negative) |
10% HLI
(Negative) |
||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Protocol | Preincubation | Preincubation | Preincubation | Preincubation | Preincubation | Preincubation | ||||||
ug/Plate | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM | Mean | ±SEM |
0 |
16 | .7 | 16 | 2.1 | 24 | 3.9 | 31 | 3.6 | 31 | 0 | 30 | 3.2 |
1 |
14 | 2.6 | ||||||||||
3 |
16 | 1.2 | 13 | .6 | 32 | 4.1 | 32 | 3.5 | ||||
10 |
20 | 2.3 | 15 | .7 | 25 | 5.7 | 29 | 1.5 | 36 | 2 | 35 | 4.3 |
33 |
22 | 3.9 | 11 | 2.3 | 27 | 3.8 | 26 | 2.3 | 32 | 1.3 | 35 | 1.8 |
100 |
17 | 4.7 | 13 | 2.5 | 29 | 2 | 31 | 3.5 | 29 | .6 | 35 | 2.2 |
333 |
8 | 2.5 | 24 | 6.3 | 34 | 2.2 | 25 | 3 | 39 | .3 | ||
1000 |
29 | 2.5 | 28 | 1.8 | ||||||||
Positive Control | 830 | 6 | 596 | 22.2 | 414 | 16.7 | 409 | 8.5 | 827 | 37.4 | 1072 | 104.4 |
HLI = induced male Syrian hamster liver S9
Applicant's summary and conclusion
- Conclusions:
- In an Ames test , test chemical dissolved in dimethyl sulfoxide from doses 0 - 1000 micrograms per plate was not mutagenic in Salmonella typhimurium strain TA 100, TA1535, TA1537 and TA98 with and without addition of S9 liver fractions from Aroclor induced hamsters and rats.
- Executive summary:
Genetic toxicity in vitro study was assessed for test chemical. For this purpose AMES test was performed .The test material was exposed to Salmonella typhimurium TA100, TA1535, TA98 and TA1537in the presence and absence of metabolic activation S9. The concentration of test material used in the presence and absence of metabolic activation were 0 ,1,3,10,33,100,333and 1000 µg/plate. No mutagenic effects were observed in all strains, in the presence and absence of metabolic activation. Therefore test chemical was considered to be non-mutagenic in Salmonella typhimurium TA100, TA1535, TA98 and TA1537by AMES test. Hence the substance cannot be classified as gene mutant in vitro.
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