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EC number: 221-641-4
CAS number: 3173-72-6
Table 1: Summary of the LLNA/IMDS results (means of 6 animals per group)
Dose 2 %
Dose 10 %
Dose 50 %
weight of draining lymph nodes
cell count in draining lymph nodes
Ear swelling in 0.01 mm on day 4 (index)
Ear weight in mg / 8 mm diameter punch on day 4 (index)
significant increase (p ≤ 0.05)
The "positive level" of ear swelling which is 2x10-2 mm increase,
i.e. about 10% of the control values, has been exceeded in all dose
groups. A significant increase compared to vehicle treated animals
regarding ear swelling and ear weights was detected in all dose groups.
An increase in this parameter would point to an acute irritating
(inflammatory) response. However, such an irritating property is also
combined with a strong skin sensitizing potential of a test compound.
The body weights of the animals were not affected by any treatment.
(NDI) was investigated in the modified local lymph node assay
(LLNA-IMDS) on female mice according to OECD TG 429. Concentrations of 0
(vehicle control), 2, 10 and 50% formulated in acetone/olive oil (4:1)
were tested. The results show that NDI has a sensitizing potential in
mice after dermal application. Compared to vehicle treated animals there
was a significant increase regarding the weights of the draining lymph
nodes and the cell counts in all dose groups. The
corresponding cell count indices were 4.06, 4.15 and 4.42 exceeding the
"positive level " of index 1.4. A significant increase
compared to vehicle treated animals regarding ear swelling and ear
weights was detected in all dose groups. An increase in this parameter would
point to an acute irritant (inflammatory) response. However, such an
irritant property is also combined with a strong skin sensitizing potential of
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