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Diss Factsheets

Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1977-06 to 1978-05
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study well documented, sufficient information about test material, test organisms

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978
Reference Type:
publication
Title:
Acute inhalation toxicity of decalin in rodents
Author:
Kinkead ER, Wolfe RE, Bunger SK and Doyle RL
Year:
1977
Bibliographic source:
Acute Toxic Data 1, 215

Materials and methods

Principles of method if other than guideline:
Acute inhalation toxicity similar to OECD 403
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Decahydronaphthalene
EC Number:
202-046-9
EC Name:
Decahydronaphthalene
Cas Number:
91-17-8
Molecular formula:
C10H18
IUPAC Name:
decahydronaphthalene
Details on test material:
decahydronaphthalene (neat), no data on purity or isomer ratio

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS:
Species/ Strain: rats, Sprague-Dawley
Sex: male
Source: Harlan Industries Inc.

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
not specified
Details on inhalation exposure:
9 l glass chamber
Analytical verification of test atmosphere concentrations:
yes
Remarks:
hydrocarbon analyser
Duration of exposure:
4 h
Concentrations:
980, 820, 785, 625, 375 ppm
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- exposure to measured concentrations of the test substance to determine an 4 hour LC 50
- Duration of observation period following administration: 14 days
Statistics:
Finney's probit analysis

Results and discussion

Preliminary study:
Exposure of 5 male rats each to saturated vapours for 4, 2 and 1-hour resulted in death of 5, 5 and 2 rats respectively. The rats were hyperactive early in the exposure but after approximately 40 minutes exhibited tonic convulsions, tremors and prostration. Rats that survived two hours or more of exposure were paralysed in the posterior half of the body and this paralysis persisted for several days. None of the paralysed rats survived the 14-day postexposure observation period.
Effect levelsopen allclose all
Key result
Sex:
male
Dose descriptor:
LC50
Effect level:
710 ppm
Based on:
test mat.
95% CL:
> 619 - < 816
Exp. duration:
4 h
Key result
Sex:
male
Dose descriptor:
LC50
Effect level:
4.08 mg/L air
Based on:
test mat.
Exp. duration:
4 h
Remarks on result:
other: calculated
Mortality:
All 5 animals died at 980 ppm, dose dependend decrease of mortality with non mortalities at 375 ppm
Clinical signs:
other: Animals displayed tremour and convulsions followed by prostration. Most survivours displayed hind quarter paralysis
Gross pathology:
Mild to severe congestion of the lungs with occasional areas of atelectasis was noted in animals that died. Reticulation of the liver and pale coloration of most organs was a common finding at all concentration levels.

Any other information on results incl. tables

 




































Concentration ppm



Concentration mg/L



Mortality



980



5.55



5/5



820



4.65



4/5



785



4.45



2/5



625



3.54



2/5



375



2.12



0/5


Applicant's summary and conclusion

Conclusions:
LC50 was determiend at 710 ppm ( with a 95% confidence intervall of 619-816 ppm). This corresponds to 4.08 mg/L air.
Executive summary:

Male Sprague-Dawley rate (5 per group) a were exposed for 4 hours to varying concentrations of Decalin vapor (375 -980 ppm). Animals were exposed in a 9-liter glas chamber and observed for 14-days post exposure. Chamber concentrations were analyzed using a hydrocarbon analyzer. LC50 was calculated using Finney's probit analysis method. Reticulation of the liver and pale coloration of most organs was a common finding at all concentration levels. 


Animals displayed tremors and convulsions followed by prostration. Most survivors displayed hind quarter paralysis. Gross pathologic examination of animals that died showed mild to severe congestion of the lungs with occasional areas of atelectasis.