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Toxicity to aquatic algae and cyanobacteria

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The acute toxicity of 'Reaction mass of benzyl 2-ethylhexyl adipate and bis(2-ethylhexyl) adipate and dibenzyl adipate' to alga (Desmodesmus subspicatus) was tested. No toxic effects against algae were observed at a limit effective loading of 100 mg/L, corresponding to a measured concentration of < 0.131 mg/L, which reflects the maximum water solubility under exposure conditions.

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A study was performed to assess the adverse effects of 'Reaction mass of benzyl 2-ethylhexyl adipate and bis(2-ethylhexyl) adipate and dibenzyl adipate' on the growth rate (= rate of increase in cell density with time) of the planktonic freshwater algal species Desmodesmus subspicatus (former name: Scenedesmus subspicatus) over several generations.

The study was conducted in accordance with Commission Regulation (EC) No 761/2009 amending Regulation No 440/2008, Method C.3 ‘Freshwater Alga and Cyanobacteria, Growth inhibition test’ (2009) which is equivalent to OECD Guideline for Testing of Chemicals No. 201 (2006).

Exponentially growing algal cells were exposed for a period of 72 hours to a limit test concentration of nominally 100 mg/L of 'Reaction mass of benzyl 2-ethylhexyl adipate and bis(2-ethylhexyl) adipate and dibenzyl adipate' dissolved in dilution water. Auxiliaries used to prepare the test media were an ultrasonic bath, a magnetic stirrer, a folded filter and an aseptic filter. The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth rate (index r), relative to control cultures grown under identical conditions.

No toxic effects against algae were observed at a limit effective loading of 100 mg/L, corresponding to a measured concentration of < 0.131 mg/L, which reflects the maximum water solubility under exposure conditions.

The results are expressed in terms of Effective Loadings (EL). After 0 and 72 hours the values were below the quantification limit of the HPLC analysis (0.131 mg/L).

'Reaction mass of benzyl 2-ethylhexyl adipate and bis(2-ethylhexyl) adipate and dibenzyl adipate' consists of three components, therefore a Water Accommodated Fraction (WAF) was used to test effects at a limit effective loading rate of 100 mg/L.