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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study according to German national standards.
Qualifier:
according to
Guideline:
other: DIN 38412; part 15
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of 1g/L was prepared in destilled water by adding the test material to the water. The test concentrations were achieved by dilution of the stock solution.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): the test solution was clear up to the 4.64 mg/L treatment. Starting from the 10 mg/L treatment the test solution was turbid.
Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Source: Fischzucht Eggers, Hohenwestedt, Germany
- Length at study initiation (length definition, mean, range and SD): 4-6 cm

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
20 °C
pH:
7.7 - 8.0
Dissolved oxygen:
7.3 - 8.6 mg/L
Nominal and measured concentrations:
Based on the results of a range finding study, the following concentrations (nominal) were tested: 0, 2.15, 4.64, 10.0, 21.5, and 46.4 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: 10 L aquaria
- Aeration: weak
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH O2 content and temperature were measured after 1,4,24,48,72 and 96h.

EFFECT PARAMETERS MEASURED: Mortality and annormal symptoms were recorded after 1,4,24,48,72 and 96h
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
14.66 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: geometric mean calculation (0% effect at 10 mg/L and 100% at 21.5 mg/L)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
21.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
From the test concentration 10 mg/l onwards, the test solutions were cloudy.
Nominal                   Dead fish after
conc. 1h 24h 48h 72h 96h
[mg/l]
-----------------------------------------------------------
2.15 0/10 0/10 0/10 0/10 0/10
4.64 0/10 0/10 0/10 0/10 0/10
10.00 0/10 0/10 0/10 0/10 0/10
21.50 0/10 10/10 10/10 10/10 10/10
46.40 10/10 10/10 10/10 10/10 10/10

control 0/10 0/10 0/10 0/10 0/10
-----------------------------------------------------------

Intoxication symptoms:
----------------------
no abnormalities
Validity criteria fulfilled:
not specified
Conclusions:
The test substance showed to be harmful to freshwater fish.

Description of key information

LC50(96h) = 14.6 mg/L (nominal) for Leuciscus idus (DIN 38412)

Key value for chemical safety assessment

LC50 for freshwater fish:
14.6 mg/L

Additional information

The acute aquatic toxicity of the Sulfonium compound mixture was investigated in a guideline study according to DIN 38412, with the golden orfe (Leuciscus idus) as test organism (BASF 1987). After 96 hours of exposure the LC50 was approximately 14.6 mg/L nominal test substance concentration.