Pentapotassium ({[(hydroxyphosphinato)methyl](phosphonatomethyl)nitroryl}methyl)phosphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to a protocol similar to OECD 203 with GLP, however no analytical monitoring was carried out.

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

yes

Remarks:

lacks analytical monitoring and a range finding test

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION

- Method: direct addition of test material to obtain each nominal concentration

- Evidence of undissolved material (e.g. precipitate, surface film, etc): none reported

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM

- Source: Incontri con la natura,Cirie, Italy

- Length at study initiation (mean): 2.3 cm

- Weight at study initiation (mean): 0.345 g

- Method of breeding: not reported

- Feeding during test: no


ACCLIMATION

- Acclimation period: 2 months

- Acclimation conditions (same as test or not): same dilution water

- Type and amount of food: Mangime Principale JBL-JBL GmbH batch 301307 until 24 hours before start of the study

- Feeding frequency: daily

- Health during acclimation (any mortality observed): 0% after 7 days of acclimation

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

40 mg/L as CaCO3 (dilution water)

Test temperature:

20⁰C

pH:

range: 6.8 to 7.6 in the controls and 7.4 to 8.5 in exposure vessels. The pH decreased with time.

Dissolved oxygen:

range: 8.0 to 9.0 mg/l

Nominal and measured concentrations:

Nominal concentrations test substance: 0, 1000, 1200, 1440, 1728 and 2074 mg/l. (Equivalent to:
Nominal concentrations ATMP n oxide xK [43%]: 0, 430, 515, 620, 745 and 890 mg/L
Nominal concentration ATMP n oxide acid [27%]: 0, 270, 325, 390, 465 and 560 mg/L)

Details on test conditions:

TEST SYSTEM

- Test vessel: tanks

- Material, size, fill volume: plastic, 30x36x24 cm filled with 10 L dilution water

- Aeration: not reported

- Renewal rate of test solution (frequency): 24 hours

- No. of organisms per vessel: 10

- No. of vessels per concentration (replicates): 1

- No. of vessels per control (replicates): 1

- Biomass loading rate: ca. 0.345 g/L


TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: from the municipal main water system

- Metals and Pesticides: within the acceptance limits of water quality defined in EEC Directive 80/778

- Culture medium different from test medium: no

- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS

- Adjustment of pH: the pH was adjusted at the start of the study in vessels to pH 6-8.5 with 37% HCl solution.

- Photoperiod: 12 to 16 hours light

- Light intensity: not reported


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality was measured every 24 hours from the start of the study. Behavioural of physiological abnormalities were also recorded at the same time.


TEST CONCENTRATIONS

- Spacing factor for test concentrations: 1.2

- Range finding study: not conducted

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1 152 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

495 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Remarks:

active salt

Basis for effect:

mortality (fish)

Remarks on result:

other: Calculated by reviewer

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

310 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Remarks:

active acid

Basis for effect:

mortality (fish)

Remarks on result:

other: Calculated by reviewer

Details on results:

- Behavioural abnormalities: none observed

- Abnormal responses: none observed

- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none

- Effect concentrations exceeding solubility of substance in test medium: no

Reported statistics and error estimates:

No details reported. LC50 values as active acid and active salt have been calculated by the reviewer from the reported LC50 value of the test material of 1152 mg/L.

Sublethal observations / clinical signs:

Table 1. Cumulative mortality results.

Nominal concentrations TS (mg/L)	# dead animals
	24 h	48 h	72 h	96 h	% mortality
Control	0	0	0	0	0
1000	1	1	1	1	10
1200	0	0	2	5	50
1440	2	4	6	7	70
1728	1	5	7	8	80
2074	5	6	8	9	90

Validity criteria fulfilled:

yes

Conclusions:

96 h LC50 values of 495 mg active salt/L, and 310 mg active acid/L (as calculated by the reviewer), have been determined for the effects of the test substance on the mortality of the freshwater fish Brachydanio rerio. Although 100% mortality was not recorded, it was possible to derive an LC50.

Description of key information

96 h LC₅₀ 310 mg active acid/L, Brachdanio rerio, (reliability 2, RBM 1991)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

310 mg/L

Additional information

A 96 h LC₅₀ value of 495 mg active salt/L, and 310 mg active acid/L, has been determined for the effects of the test substance on the mortality of the freshwater fish Brachydanio rerio (RBM 1991). Although 100% mortality was not recorded, it was possible to derive an LC₅₀. The study was conducted according to EC test method C.1, however no analytical monitoring was carried out. This is the only study available with the substance and has been selected as key.